There is a growing recognition that neuroinflammation plays a crucial role not only in classic neuroinflammatory disorders like multiple sclerosis (MS) but also in a wide range of neurodegenerative conditions, including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and amyotrophic lateral sclerosis (ALS). Research has shown that irregular or dysfunctional activation of neuroinflammatory pathways in these conditions significantly contributes to demyelination and neurodegeneration. There is also increasing evidence that inflammatory responses in the central nervous system (CNS) may play a part in the development of psychiatric disorders, such as major depressive disorder and schizophrenia. 

Given the rising significance of neuroinflammation, this collection of methods aims to provide standardized and reproducible protocols for studying the inflammatory processes affecting the CNS. By curating clear, accessible, and rigorous methodologies, we hope to support the research community in conducting high-quality mechanistic studies involving neuroinflammation and accelerate discoveries that enhance our understanding of its role in neurological and psychiatric disorders.