Since their emergence in 2011, cell-derived nanoparticles (CNPs) have attracted growing attention due to their unique biological properties and broad potential in therapeutic and diagnostic applications. From biomimetic coating strategies utilizing synthetic nanomaterials to the engineering of extracellular vesicles for enhanced therapeutic performance, CNPs offer a promising platform for targeted drug delivery across a wide range of diseases. Their intrinsic biocompatibility and capacity for customization make them one of the most dynamic and interdisciplinary areas in nanomedicine today.

Despite rapid progress, the field faces major challenges in reproducibility, standardization, and large-scale production, which currently hinder translational and clinical applications.

This Methods Collection aims to address these challenges by presenting experimental protocols that enable researchers to develop, characterize, and upscale CNPs more effectively. By bringing together the latest techniques and interdisciplinary approaches, this collection will serve as a comprehensive resource to standardize methodologies across the field, facilitating comparison of results and accelerating innovation.

Topics of interest include, but are not limited to:

• Novel biomimetic coating strategies for synthetic nanomaterials
• Isolation and purification methods for CNPs (including extracellular vesicles)
• Engineering strategies to enhance nanoparticle functionality
• Scalable production and quality control of CNPs
• Innovative therapeutic and diagnostic applications

This collection ultimately aims to promote methodological transparency and reproducibility, enabling the research community to establish reliable standards for the next generation of cell-derived nanotechnologies.