The proposed Topical Collection addresses the need for standardization and transparency in preclinical metabolic research. Metabolic diseases such as obesity, type 2 diabetes, and MASLD/MASH represent major global health challenges. Yet variability in animal models, phenotyping techniques, and intervention protocols limits reproducibility and cross-study comparison.

This collection aims to provide methodological resources for researchers conducting in vivo studies of metabolic diseases by presenting visually demonstrated, step-by-step protocols for:

• Establishment and validation of metabolic disease models: including obesity, insulin resistance, type 2 diabetes, hypertension, and dyslipidemia
• Metabolic phenotyping: including glucose and insulin tolerance tests, indirect calorimetry, and clamp techniques
• Administration and assessment of interventions: including pharmacological treatments (injection, gavage, incorporation), exercise protocols (voluntary, forced, electrical stimulation), and nutraceutical and dietary interventions
• Evaluation of treatment efficacy: through integrated physiological and metabolic readouts

By consolidating standardized methodologies and promoting reproducible experimental practices, this collection aims to enhance cross-study comparability, support translational research, and advance the development of effective therapeutic strategies for metabolic diseases.