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TOPICAL COLLECTIONS

Standard and Novel Methods in the Study of Corticogenesis

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Isabel Garay

Isabel Garay

Cardiff University

Dr. Isabel Martinez Garay is a Senior Lecturer in Neuroscience in the School of Biosciences, Cardiff University. Her research aims at elucidating how cross-talk between cells shapes the developing brain by influencing processes such as neurogenesis, migration, and synaptogenesis. She is particularly interested in cell-cell adhesion molecules, both as mediators of physical contact between cells and as potential signaling hubs.  Her research combines different in vivo, ex vivo and in vitro techniques, including the use of transgenic animals, in utero electroporation, ESC-derived neurons, calcium imaging, and RNA sequencing, to study the roles of different cell adhesion proteins during corticogenesis. Prior to joining Cardiff University, Dr. Martinez Garay carried out postdoctoral research, first at the Scripps Research Institute in La Jolla as a Fullbright and a CIRM fellow, and later at the University of Oxford.  

Collection Overview

The cerebral cortex is the seat of higher brain function and plays a key role in memory, attention, thought, perception and language. Development of this cerebral structure is a complex process in which different steps need to be tightly regulated and coordinated in order to obtain a mature, six-layered structure. Progenitors at the ventricular zone generate specific types of neurons at the appropriate time and in correct numbers. These neurons then migrate into the nascent cortical plate while simultaneously extending their axons and, once they arrive at their final position in the right layer, they start to elaborate the dendritic arbor and establish synaptic contacts with specific targets. Defects in cortical development have long been associated with disorders displaying anatomical abnormalities, like microcephaly, lissencephaly or periventricular heterotopia. In addition, development of the cerebral cortex is also disrupted in other neurological conditions that do not manifest with obvious anatomical changes, such as ASD, schizophrenia or cognitive impairment. 

Cortical development is therefore an area of very active research, which has progressed rapidly in recent times. This collection aims to include standard and novel methods in the study of corticogenesis, with a focus on approaches to target or label progenitors and neurons for lineage tracing and functional studies of neurogenesis, fate specification and neuronal migration.

 

Articles

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)
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Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

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Cited by 28

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2020

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