200 Kv Tem
200 Kv Tem - Related Videos
02:32
Sectioning, Staining and TEM Imaging of Embedded Exosomes: A Protocol To Visualize the Structural Features of Exosomes Using TEM
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2023
This video describes the technique for preparing a thin section of embedded exosomes. These sections can further be stained and imaged using transmission electron microscopy, or TEM, to study the structural features of exosomes.
09:49
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
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Cited by 13 •
2022
High-resolution cryo-EM maps of macromolecules can be also achieved by using 200 kV TEM microscopes. This protocol shows the best practices for setting accurate optics alignments, data acquisition schemes, and selection of imaging areas that are all essential for the successful collection of high-resolution datasets using a 200 kV TEM.
08:28
In situ TEM of Biological Assemblies in Liquid
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Cited by 8 •
2013
Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.
09:17
An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis
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Cited by 9 •
2016
Here, we present a protocol to prepare samples with low cell numbers for transmission electron microscopy (TEM) analysis.
10:56
An Unbiased Approach of Sampling TEM Sections in Neuroscience
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Cited by 3 •
2019
We introduce a novel workflow for electron microscopy investigations of brain tissue. The method allows the user to examine neuronal features in an unbiased fashion. For elemental analysis, we also present a script that automatizes most of the workflow for randomized sampling.
FAQs
At this accelerating voltage, the electron beam can support high-resolution visualization of structures that are too small for light microscopy. The setting therefore determines the operating context in which nanoscale features can be examined, while the final image still depends on electrons transmitted through the specimen and focused by electromagnetic lenses.
Image formation reflects differences in how electrons interact with the specimen. Regions that differ in electron scattering and density contribute differently to the transmitted beam, producing image variation after the electromagnetic lenses focus those electrons. This contrast allows investigators to evaluate visible morphology and organization rather than relying only on a biochemical composition measurement.
An ultrathin specimen allows the electron beam to pass through the material so transmitted electrons can be collected and focused into an image. That transmission-based requirement distinguishes the technique from observations that do not depend on electrons crossing the sample. In biochemical work, it makes nanoscale structural features accessible for direct visual assessment.
The workflow begins with an ultrathin specimen, places it in the path of a 200-kilovolt electron beam, and uses electromagnetic lenses to focus transmitted electrons. The resulting image is interpreted through differences in electron scattering and density. This sequence connects specimen preparation, electron transmission, lens-based image formation, and structural assessment.
Purified proteins, macromolecular assemblies, membranes, and cellular ultrastructure are all identified as relevant specimen types. Examining these materials can reveal morphology, organization, and specimen quality at a scale useful to biochemistry. The appropriate choice depends on whether the study focuses on an isolated molecular system, a membrane, or ultrastructural organization in cells.
It adds nanoscale architectural information to biochemical and structural analyses. By showing morphology and organization, the images can help connect molecular composition with the way material is arranged in space. This complementary role is especially useful when researchers need to relate what a sample contains to the architecture or quality visible in the specimen.