An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis

The overall goal of this procedure is to prepare samples with low cell numbers to perform TEM analysis in an efficient and reproducible manner. This method can help to reveal all structures of real cells and a better physiological settings in the biomedical field. The main advantage of this technique is that both qualitative and quantitative results can be obtained from low cell numbers in reproducible manner.

To begin, use facts to sort 10, 000 hematopoietic stem cells, or HSC's into a 1.5 mL microcentrifuge tube. Containing 600 microliters of isocoves modified dulbecco medium with 10%FBS. Using a centrifuge with swing out buckets, spin down the sorted HSC's at 1, 000 times G for 10 minutes.