Carbon-13 Nitrogen-15
Carbon-13 Nitrogen-15 - Related Videos
10:41
Carbon and Nitrogen Analysis of Environmental Samples
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Elemental Analysis is a method used to determine elemental composition of a material. In environmental samples such as soils, scientists are particularly interested in the amounts of two ecologically important elements, nitrogen and carbon. Elemental analysis by the flash combustion technique works by oxidizing the sample with a catalyst through combustion in a high-temperature chamber. The products of combustion are...
10:16
Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
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Cited by 39 •
2014
This method explains how to build and operate a continuous 13C and 15N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.
07:11
Biofilm Removal Using Carbon Dioxide Aerosols without Nitrogen Purge
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Cited by 2 •
2016
Biofilms on surfaces can be effectively and rapidly removed by using a periodic jet of carbon dioxide aerosols without a nitrogen purge.
01:10
Carbon-13 (¹³C) NMR: Overview
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2024
Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...
08:44
Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
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Cited by 2 •
2020
A microplot design for 15N tracer research is described to accommodate multiple in-season plant and soil sampling events. Soil and plant sample collection and processing procedures, including grinding and weighing protocols, for 15N analysis are put forth.
FAQs
Carbon-13 and nitrogen-15 preserve the chemical behavior of the more abundant carbon and nitrogen isotopes, so labeled nutrients can participate in biochemical processes rather than acting as unrelated tracers. Their different mass and nuclear properties make the labeled atoms distinguishable to analytical methods. This combination lets researchers follow atom movement while still examining native biochemical transformations.
Mass spectrometry distinguishes labeled molecules through their mass, whereas nuclear magnetic resonance spectroscopy detects information associated with the isotopes’ nuclear properties. The methods therefore provide complementary ways to identify incorporation and examine molecular structure. Selecting one or both allows a study to connect labeled-atom placement with structural or pathway-level questions.
Label incorporation links an introduced nutrient to the cellular molecules that later contain its carbon or nitrogen atoms. Detecting that incorporation provides evidence about where those atoms move through a metabolic pathway and how they contribute to products. In biochemistry, this turns a proposed pathway into an experimentally traceable flow of atoms.
A typical experiment begins by introducing a 13C- or 15N-labeled nutrient into the biochemical system. Researchers then analyze resulting molecules with mass spectrometry or nuclear magnetic resonance spectroscopy and interpret the detected label in relation to the molecules examined. This workflow can reveal whether supplied carbon or nitrogen entered cellular molecules and support pathway or structural analysis.
Protein studies use these labels to obtain information about structure and dynamics, not only about nutrient movement. The labeled atoms provide detectable features that can be examined with the available analytical methods, allowing researchers to investigate how a protein is organized and how it changes over time. This connects molecular composition with protein behavior.
Labeling supports investigations of biomolecular interactions and the contribution of atoms to cellular molecules. By tracking where labeled carbon or nitrogen appears, researchers can relate molecular partners or products to the underlying biochemical process. The resulting evidence helps clarify how molecules interact and how nutrient-derived atoms become part of cellular biochemistry.