Duplex Dna
Duplex Dna - Related Videos
11:42
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
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2012
Crystal structure of protein–DNA complexes can provide insight into protein function, mechanism, as well as, the nature of the specific interaction. Here, we report how to optimize the length, sequence and ends of duplex DNA for co-crystallization with Escherichia coli SeqA, a negative regulator of replication initiation.
08:45
Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers
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2025
In this video, we demonstrate the duplex digital PCR (ddPCR) technique — a modification of the traditional PCR technique that is useful in detecting two different genetic markers simultaneously. A single PCR reaction is partitioned into nanoliter-sized emulsified droplets that are independently amplified, and the detection of differently colored fluorescence amplification signals from the fraction of droplets is used to compute the initial concentration of the target sequences.
04:10
Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes
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2025
In this video, we demonstrate the procedure to determine the helicase activity of a target protein to unwind the biotin-labeled dsRNA substrate. The activity of the enzyme was identified by analyzing the electrophoretic mobility shift, followed by a chemiluminescence assay using chemiluminescent enzyme-conjugated streptavidin.
12:14
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
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Cited by 14 •
2016
This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.
07:38
DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
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Cited by 2 •
2017
This protocol describes the characterization of DNA polymerase synthesis of modified DNA through observation of changes to near-infrared fluorescently labeled DNA using gel electrophoresis and gel imaging. Acrylamide gels are used for high resolution imaging of the separation of short nucleic acids, which migrate at different rates depending on size.
FAQs
Antiparallel orientation places the two strands in opposite directions, while complementary pairing links each position to a predictable partner. During replication, that arrangement means either existing strand can guide formation of a matching sequence. The result is a structural basis for copying genetic information accurately, rather than relying on an independent reconstruction of the entire sequence.
Hydrogen bonds between paired bases help maintain the duplex, while the pairing rules preserve sequence correspondence between strands. This combination is biochemically important because the molecule must remain sufficiently stable to store information yet retain an organized sequence that proteins can recognize. The same correspondence allows sequence information to be interpreted through the opposing strand.
Sequence-specific recognition means that proteins can distinguish particular arrangements of bases rather than interacting with duplex DNA as an undifferentiated molecule. Such recognition connects DNA structure with gene expression: proteins identify relevant sequences, and their interactions can influence how genetic information is used. In biochemistry, this links molecular structure to regulation without changing the underlying genetic storage role.
A mutation changes the nucleotide sequence within DNA and can therefore affect complementary relationships or sequence-specific recognition. Such changes may influence how information is copied, repaired, or used in gene expression. Because duplex DNA stores information through an ordered sequence, even a localized change can be studied in relation to heredity, molecular recognition, and altered biological outcomes.
Its complementary strands connect information storage with transmission. A strand can serve as a template, so sequence information can be reproduced while retaining the pairing relationship that characterizes the molecule. This provides the biochemical foundation for heredity: information encoded in one generation's DNA can be copied and passed on, while sequence differences provide a molecular basis for mutation.
Duplex DNA is relevant to biotechnology and molecular diagnostics because its sequence and base-pairing behavior provide information that can be recognized and interpreted. Studying its structure and interactions helps researchers examine genetic information, sequence-specific molecular recognition, and changes associated with mutation or repair. These applications extend the topic beyond structure into analysis of biological information.
In biochemistry, duplex DNA is examined as both a molecule and an interaction platform. Its nucleotide arrangement determines complementary pairing, while its sequence supports recognition by proteins. This perspective connects chemical forces, molecular structure, replication, repair, and gene expression. It helps explain how the physical properties of a nucleic acid produce cellular information-processing functions.