Nitrogen Cavitation
Nitrogen Cavitation - Related Videos
08:24
Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
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Cited by 15 •
2017
Here we present protocols for detergent-free homogenization of cultured mammalian cells based on nitrogen cavitation and subsequent separation of cytosolic and membrane-bound proteins by ultracentrifugation. This method is ideal for monitoring the partitioning of peripheral membrane proteins between soluble and membrane fractions.
14:22
Activating Molecules, Ions, and Solid Particles with Acoustic Cavitation
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Cited by 6 •
2014
Acoustic cavitation in liquids submitted to power ultrasound creates transient extreme conditions inside the collapsing bubbles, which are the origin of unusual chemical reactivity and light emission, known as sonoluminescence. In the presence of noble gases, nonequilibrium plasma is formed. The "hot" particles and the photons generated by collapsing bubbles are able to excite species in solution.
01:49
The Nitrogen Cycle
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2019
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
11:14
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
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Cited by 8 •
2017
A microfluidic chip was fabricated to produce pairs of gold dots for tandem bubble generation and fibronectin-coated islands for single-cell patterning nearby. The resultant flow field was characterized by particle image velocimetry and was employed to study various bioeffects, including cell membrane poration, membrane deformation, and intracellular calcium response.
10:41
Carbon and Nitrogen Analysis of Environmental Samples
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Elemental Analysis is a method used to determine elemental composition of a material. In environmental samples such as soils, scientists are particularly interested in the amounts of two ecologically important elements, nitrogen and carbon. Elemental analysis by the flash combustion technique works by oxidizing the sample with a catalyst through combustion in a high-temperature chamber. The products of combustion are...
FAQs
The critical event occurs after nitrogen has dissolved into the cell suspension under high pressure. Rapid decompression allows the dissolved gas to expand inside and around the cells, producing shear forces that rupture cell membranes. This pressure-to-expansion sequence provides a controlled physical mechanism for opening cells without relying primarily on intense mechanical grinding or homogenization.
Nitrogen Cavitation can limit two sources of structural damage: heat generation and excessive mechanical stress. By using pressurized gas followed by controlled decompression, the method may release cellular contents while better maintaining native proteins, enzymes, organelles, and other structures. Preserving these components is important when their function or organization must be examined after lysis.
Pressure, nitrogen dissolution, and the rate of decompression are central variables because they control the gas expansion that generates membrane-disrupting shear forces. The biological material also matters, since the method is applied to cell suspensions from cultured cells or tissues. Controlling these conditions helps balance effective membrane rupture with preservation of native cellular components.
A typical workflow begins with a suspension prepared from cultured cells or tissue. Nitrogen is then introduced under high pressure so it dissolves into the suspension, followed by rapid decompression to trigger gas expansion and membrane rupture. The resulting disrupted material can support extraction of soluble proteins, enzymes, or subcellular organelles for biochemical analysis.
Researchers may choose Nitrogen Cavitation when they need cellular disruption while minimizing heat and mechanical damage. This consideration is especially relevant for experiments focused on protein function, enzyme recovery, organelle preservation, or native cellular organization. The technique therefore suits biochemical studies in which the extracted material must retain meaningful structural or functional properties after lysis.
The method can provide access to soluble proteins, enzymes, and subcellular organelles from cultured cells or tissues. These materials support investigations of protein function, cellular organization, and biochemical pathways. Because the process can preserve native structures, researchers can use the resulting extracts to examine cellular components in a condition that remains closer to their original organization.