Biomimetic Microfluidic Assay
Biomimetic Microfluidic Assay - Related Videos
09:39
A Microfluidic Model of Biomimetically Breathing Pulmonary Acinar Airways
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Cited by 17 •
2016
Soft-lithography was utilized to produce a representative true-scale model of pulmonary alveolated airways that expand and contract periodically, mimicking physiological breathing motion. This platform recreates respiratory acinar flows on a chip, and is anticipated to facilitate experimental investigation of inhaled aerosol dynamics and deposition in the pulmonary acinus.
12:22
Biomimetic Materials to Characterize Bacteria-host Interactions
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Cited by 8 •
2015
Bacterial attachment to host cells is a key step during host colonization and infection. This protocol describes the generation of polymer-coupled recombinant adhesins as biomimetic materials which allow analysis of the contribution of individual adhesins to these processes, independent of other bacterial factors.
10:35
Studies of Bacterial Chemotaxis Using Microfluidics - Interview
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Cited by 1 •
2007
10:53
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
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Cited by 10 •
2017
This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.
08:50
Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
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2026
This work describes a microfluidic assay that uses shear stress to trigger thrombus formation in blood and characterizes the thrombus by multiple dimensions of readout. The assay can measure the prothrombotic tendency of blood samples, and is thus useful in disease diagnosis, drug discovery, as well as basic mechanistic studies related to thrombosis.
FAQs
Its microchannel network regulates fluid movement, concentration gradients, and the duration or extent of cell exposure to relevant conditions. These controls let investigators examine how cells respond within a defined local environment rather than under broadly mixed conditions. In bioengineering studies, that spatial and exposure control supports quantitative measurements of cellular behavior and biochemical responses.
Engineered materials and structures provide features that resemble tissue architecture and local physiological conditions. This added biological context can make cellular responses more representative than results from conventional assays that reproduce fewer environmental features. The approach is therefore useful when researchers need to evaluate transport, toxicity, or cell behavior in an environment designed to reflect aspects of living tissue.
Fluid flow, concentration gradients, cell exposure, and the design of the engineered environment are central variables. Changing any of these features can alter the conditions cells encounter and therefore influence measured transport, toxicity, behavior, or biochemical responses. Controlling them systematically helps bioengineers connect an observed outcome with a defined microscale condition rather than an uncontrolled sample environment.
A useful experiment must establish controlled microscale fluid handling while incorporating biological design features relevant to the question under study. The device should provide defined flow, concentration, and cell-exposure conditions, together with materials or structures that represent important tissue characteristics. This combination allows small sample volumes to generate quantitative information under deliberately selected environmental conditions.
Researchers may choose it for disease modeling and drug screening when conventional assays do not capture enough of the relevant tissue environment. By reproducing selected architectural and physiological features, the assay can provide a more biologically relevant setting for observing cellular and biochemical responses. Its controlled microscale format also supports quantitative analysis while using small sample volumes.
The assays can support quantitative analysis of cell behavior, transport, toxicity, and biochemical responses. These readouts help investigators characterize how cells and substances respond to controlled local conditions, providing evidence for biological modeling and technology development. In broader bioengineering applications, the resulting measurements can inform advanced diagnostic and therapeutic technologies as well as screening and disease-modeling studies.