Calcium Phosphate Ink

Calcium phosphate ink is a bioengineering material formulated from calcium phosphate particles or precursors that can be deposited into organized three-dimensional structures, particularly for bone-related research. During extrusion-based printing, the ink’s rheology allows it to flow through a nozzle under pressure, retain its shape after deposition, and, in some formulations, harden through cementation or other setting reactions. Its mineral composition resembles the inorganic phase of bone, making these inks useful for fabricating porous scaffolds, studying cell-material interactions, and developing strategies for bone repair. Printability, mechanical stability, degradation, and biological compatibility help determine their performance.

Calcium Phosphate Ink - Related Videos

Education

JoVE Core - Anatomy and Physiology

Roles of Electrolytes: Calcium and Phosphate

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2024

Calcium and phosphate are essential electrolytes in the human body, with calcium being the most abundant mineral. Around 99% of the body's calcium is stored in the skeleton and teeth, forming a crystal lattice of mineral salts in combination with phosphates. Calcium plays crucial roles in various bodily functions such as blood clotting, neurotransmitter release, muscle tone maintenance, and nervous and muscle tissue excitability. The calcium concentration in blood plasma is primarily regulated...

Research

JoVE EoE - Assay Techniques

Resorption Pit Assay: An In Vitro Technique to Quantify Calcium Resorption Activity of Mature Osteoclasts using Calcium Phosphate-Coated Culture Plates

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2025

In this video, we describe an in vitro osteoclastic resorption assay on calcium phosphate-coated cell culture plates cultured with human peripheral blood mononuclear cell-derived osteoclasts. The osteoclasts form a characteristic actin ring on the calcium phosphate-coated matrix of the cell culture plate and degrade the matrix within the actin ring region to release calcium and phosphate ions, resulting in the formation of distinct resorption pits within the degraded matrix.

Calcium Phosphate Transfection of Primary Hippocampal Neurons

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Cited by 31 •

2013

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

Research

JoVE Journal - Bioengineering
Free Sample

Planar and Three-Dimensional Printing of Conductive Inks

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Cited by 58 •

2011

Planar and three-dimensional printing of conductive metallic inks is described. Our approach provides new avenues for fabricating printed electronic, optoelectronic, and biomedical devices in unusual layouts at the microscale.

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model

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Cited by 4 •

2022

This protocol describes a calcium phosphate-induced abdominal aortic aneurysm (AAA) mouse model to study the pathological features and molecular mechanisms of AAAs.

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