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Calcium Phosphate Transfection of Primary Hippocampal Neurons

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Article DOI: 10.3791/50808-v 08:22 min November 12th, 2013
November 12th, 2013



Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.


Calcium Phosphate Precipitation Transfection Primary Hippocampal Neurons Cultured Cells Hard-to-transfect Cells Protocol Astroglial Cells
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