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Neuroscience

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Calcium Phosphate Transfection of Primary Hippocampal Neurons
 

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Article doi: 10.3791/50808
November 12th, 2013

Summary November 12th, 2013

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

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