Cellular Metabolic Impact
Cellular Metabolic Impact - Related Videos
03:10
An Assay to Study the Impact of Photodynamic Treatment with a Photosensitizer on Macrophage Metabolic Activity
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2025
Source:Uju L. Madu1, Nozethu Mjokane1, Maphori Maliehe1, Olihile M. Sebolai11Department of Microbiology and Biochemistry, University of the Free State.The video demonstrates a technique for evaluating the influence of photodynamic treatment (PDT) with a photosensitizer on the metabolic activity of murine macrophages. The cells undergo treatment with the photosensitizer followed by exposure to UV light, initiating PDT. This process triggers the photosensitizer to generate reactive oxygen species...
07:36
Autofluorescence Imaging to Evaluate Cellular Metabolism
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Cited by 15 •
2021
This protocol describes fluorescence imaging and analysis of the endogenous metabolic coenzymes, reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H), and oxidized flavin adenine dinucleotide (FAD). Autofluorescence imaging of NAD(P)H and FAD provides a label-free, nondestructive method to assess cellular metabolism.
08:20
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
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Cited by 21 •
2019
The purpose of the method presented here is to show how microenvironment microarrays (MEMA) can be fabricated and used to interrogate the impact of thousands of simple combinatorial microenvironments on the phenotype of cultured cells.
04:47
Multimodal Optical Imaging Platform for Studying Cellular Metabolism
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2025
Utilizing a multimodal platform combining label-free optical imaging modalities, we have developed a protocol for visualizing and quantifying cellular dynamics and metabolism. Through imaging via multiphoton fluorescence, second harmonic generation, and stimulated Raman scattering microscopy, we can generate a holistic overview of the cellular and molecular environment.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
FAQs
Nutrient availability and oxygen levels can shift how cells distribute metabolic flux among glycolysis, the tricarboxylic acid cycle, and amino acid synthesis. Enzyme activity and signaling pathways further influence these routes, changing energy use, biosynthetic output, nutrient consumption, or waste production. Monitoring these shifts helps reveal how an intervention or engineered environment alters cellular function.
Metabolic flux describes the movement of cellular resources through biochemical pathways. Its distribution indicates whether cells favor energy production, biosynthesis, or other demands under a given condition. Examining flux through glycolysis, the tricarboxylic acid cycle, and amino acid synthesis can show how an engineered cell responds to its environment and whether its metabolism supports the intended function.
Changes in energy use, nutrient consumption, biosynthetic activity, and waste production may indicate that cells are responding to an altered environment. The same measurements can distinguish adaptation from growth limitation when interpreted alongside changes caused by nutrient availability, oxygen levels, enzyme activity, or signaling. This makes metabolic impact a useful indicator of cellular condition.
Researchers assess the impact by measuring changes in cellular energy use, biosynthesis, nutrient consumption, and waste production after applying a biological intervention, material, or engineered environment. They can then relate those changes to metabolic pathways and cellular outcomes such as stress, adaptation, growth limitations, or performance. The comparison supports evaluation of whether the engineered condition produces the desired response.
It matters when researchers need to determine whether a material or engineered environment supports healthy cellular function or disrupts it. Metabolic measurements can reveal unfavorable stress responses, limited growth, or altered biosynthesis, while favorable patterns may help optimize conditions for cell-based manufacturing. This information supports decisions about whether an intervention is suitable for continued bioengineering development.
Measurements of energy use, nutrient consumption, biosynthesis, and waste production can show whether a scaffold or culture condition supports the desired cellular behavior. Researchers can use these outcomes to identify growth limitations or stress and to refine the engineered environment. In tissue engineering, this provides a metabolic basis for designing conditions that better support cell or tissue performance.