Chamber Implantation
Chamber Implantation - Related Videos
16:40
A New Single Chamber Implantable Defibrillator with Atrial Sensing: A Practical Demonstration of Sensing and Ease of Implantation
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Cited by 5 •
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Dual-chamber implantable cardioverter-defibrillators (ICDs) may improve detection of atrial fibrillation as well as differentiation of tachycardias. However, this advantage is undermined by complications associated with the second electrode, which is required in conventional dual chamber devices. Therefore, BIOTRONIK has developed a new electrode called the LinoxSMART S DX that, when used in conjunction with the Lumax DX ICD, offers dual-chamber detection without the risks associated with the...
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
11:20
A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord
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Cited by 15 •
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In this video, we describe a procedure for implanting a chronic optical imaging chamber over the dorsal spinal cord of a live mouse. The chamber, surgical procedure, and chronic imaging are reviewed and demonstrated.
05:54
Microbead Implantation in the Zebrafish Embryo
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Cited by 8 •
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The zebrafish is an excellent model system for genetic and developmental studies. Bead implantation is a valuable tissue manipulation technique that can be used to interrogate developmental mechanisms by introducing alterations in local cellular environments. This protocol describes how to perform microbead implantation in the zebrafish embryo.
09:55
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Cited by 17 •
2016
This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.
FAQs
The transparent window is central because it allows researchers to observe living tissue repeatedly without removing the chamber. At the same time, implantation must preserve local blood flow and tissue viability, so observed microvascular or inflammatory changes can be interpreted as ongoing biological responses rather than measurements from an isolated or nonviable preparation.
Longitudinal measurements distinguish Chamber implantation from approaches that provide only a single endpoint. Researchers can follow microvascular dynamics, inflammation, wound healing, or angiogenesis in the same subject over time. Repeated observation helps connect the progression of tissue responses with changes in an implanted device, biomaterial, or engineered tissue, while reducing reliance on comparisons between different subjects.
Stability makes the observation site accessible across repeated sessions and helps preserve the local conditions needed for meaningful measurements. If the interface does not support tissue viability or local blood flow, changes seen through the window may not represent normal tissue responses. In bioengineering studies, this stability is therefore important for evaluating inflammation, healing, vascular growth, and integration.
At a high level, the procedure requires surgically securing the chamber to the selected target tissue and establishing an accessible observation site, often with a transparent window. The implanted setup must maintain local blood flow and tissue viability during subsequent observations. This arrangement enables repeated intravital imaging rather than a single examination at the end of the study.
Measurements may include microvascular dynamics, inflammation, wound healing, and angiogenesis, as well as the integration of biomaterials or engineered tissues. Because observations can be repeated in the same subject, investigators can assess how these responses develop over time rather than relying only on a final snapshot. The resulting data connect local tissue behavior with the performance of a bioengineered intervention.
It is particularly useful when a study needs repeated access to living tissue while testing a device, biomaterial, engineered tissue, or regenerative approach. The method supports direct observation of tissue responses during development, making it relevant to regenerative therapies and drug-delivery systems. It can also help evaluate whether a material or engineered construct becomes integrated while local vascular and inflammatory responses are monitored.