Histology Processing Cassette
Histology Processing Cassette - Related Videos
11:03
Pouring and Running a Protein Gel by reusing Commercial Cassettes
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Cited by 2 •
2012
Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.
09:37
Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
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Cited by 13 •
2015
This protocol describes the detection of class 1 integrons and their associated gene cassettes in foodstuffs.
09:27
Histological Sample Preparation for Light Microscopy
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2023
Histology is the study of cells and tissues, which is typically aided by the use of a light microscope. The preparation of histological samples can vary greatly based on the inherent properties of the samples such as size and hardness as well as expected post-processing which includes planned staining techniques or other down-stream applications. As described in this video, specimen preparation typically begins with a fixation procedure to prevent degradation of the sample by naturally...
10:45
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
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Cited by 27 •
2017
This protocol describes histological analyses of the livers from zebrafish larvae that have been treated with 2% ethanol for 24 h. Such acute ethanol treatment results in hepatic steatosis and swelling of the hepatic vasculature.
05:33
Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses
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Cited by 3 •
2019
Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.
FAQs
The openings permit fixatives, dehydrating agents, clearing solvents, and molten paraffin to reach the specimen during successive processing stages. This exchange supports consistent treatment throughout the tissue while the cassette keeps the sample contained. In bioengineering studies, reliable reagent access helps preserve material for later assessment of cellular organization, architecture, and integration.
Maintaining specimen identity links the processed tissue to its original sample and experimental context. The cassette's labeling and continuous containment support traceability as the specimen moves through fixation, processing, embedding, sectioning, and staining. This is especially important when comparing engineered tissues, biomaterial responses, or multiple tissue samples prepared for microscopic evaluation.
After the processing reagents have acted on the specimen, molten paraffin enters through the cassette openings and supports embedding. The resulting paraffin-embedded tissue can then be sectioned into thin slices and stained for analysis. This sequence converts a handled specimen into prepared material suitable for examining cellular organization, tissue architecture, and tissue integration.
A labeled, perforated cassette combines reagent access with continuous physical support and sample identification. That combination reduces changes in handling between preparation stages and promotes standardized processing. Consistency matters when microscopic findings must be compared across tissue samples, engineered constructs, or biomaterial evaluations, because differences in preparation can complicate interpretation of tissue structure.
The specimen is first fixed, then exposed to dehydrating agents, clearing solvents, and molten paraffin while remaining associated with its labeled cassette. After paraffin embedding, the tissue is sectioned into thin slices and stained. These stages prepare the sample for microscopic examination while preserving traceability and supporting consistent movement through the workflow.
Bioengineers can use the workflow to evaluate engineered tissues, tissue samples, and responses to biomaterials. Processing preserves a route from sample identity to microscopic analysis, allowing investigators to examine cellular organization, tissue architecture, and integration. The approach is useful when standardized preparation is needed to compare how tissues or materials perform within an experimental study.
Following sectioning and staining, microscopic examination can reveal cellular organization and tissue architecture within the prepared sample. In bioengineering applications, the same evaluation can support assessment of how tissue integrates with an engineered structure or responds to a biomaterial. The cassette contributes to the reliability of these observations by supporting traceable, consistent preparation.
Biomaterial response studies often require tissue findings to remain connected to the correct sample and preparation history. Labeling supports that connection, while the cassette maintains the specimen through reagent exposure, paraffin embedding, sectioning, and staining. Together, these features help produce interpretable microscopic comparisons of tissue architecture, cellular organization, and integration around engineered materials.