In Vivo Integration
In Vivo Integration - Related Videos
12:17
The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo
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Cited by 18 •
2017
We present a detailed method to study human placental physiology in vivo at term. The method combines blood sampling from the incoming and outgoing vessels on the maternal and fetal sides of the placenta with ultrasound measurements of volume blood flow and placental tissue sampling.
11:38
In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice
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Cited by 7 •
2014
This video illustrates a method, using a clinical 3 T scanner, for contrast-enhanced MR imaging of the naïve mouse visual projection and for repetitive and longitudinal in vivo studies of optic nerve degeneration associated with acute optic nerve crush injury and chronic optic nerve degeneration in knock-out mice (p50KO).
05:48
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
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Cited by 7 •
2018
Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.
09:16
Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
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Cited by 36 •
2015
Ex vivo ERG can be used to record electrical activity of retinal cells directly from isolated intact retinas of animals or humans. We demonstrate here how common in vivo ERG systems can be adapted for ex vivo ERG recordings in order to dissect the electrical activity of retinal cells.
07:08
Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
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Cited by 8 •
2013
In this article we introduce fast micro-iontophoresis of neurotransmitters as a technique to investigate integration of postsynaptic signals with high spatial and temporal precision.
FAQs
Cell adhesion helps anchor the engineered construct to surrounding tissue and supports interaction between the construct and host cells. This attachment works together with extracellular matrix remodeling, which changes the local structural environment over time. If adhesion and matrix interactions are coordinated, the construct is more likely to remain stable and develop a functional connection with adjacent tissue.
Vascularization is a central component of successful integration because it helps establish a connection between the engineered construct and the host environment. Its development must occur alongside cell adhesion and extracellular matrix remodeling rather than as an isolated event. In bioengineering studies, the extent of vascularization helps researchers evaluate whether a graft or tissue construct is connecting effectively with surrounding tissue.
Immune regulation influences how the host responds to an engineered material, device, cell population, or tissue construct. That response must be coordinated with adhesion, matrix remodeling, and vascularization to support stable incorporation. Examining immune regulation helps researchers identify whether host-construct interactions are compatible with long-term performance and whether a design may require modification before further development.
A construct may remain present in the body without achieving a meaningful functional connection with surrounding tissue. Researchers therefore consider both its stability and the coordinated evidence of adhesion, extracellular matrix remodeling, vascularization, and immune regulation. This distinction is important because long-term performance depends not only on retention, but also on effective interaction with the host environment.
Researchers first place the engineered material, device, cell population, or tissue construct within a living organism, then examine how it interacts with the host environment. Evaluation focuses on stability and functional connection, with attention to cell adhesion, matrix remodeling, vascularization, and immune regulation. These observations indicate whether the engineered system is performing as intended in vivo.
These studies are used to evaluate implants, tissue-engineered grafts, drug delivery systems, and regenerative therapies. They provide information about how each engineered solution behaves in a host rather than only in laboratory settings. The resulting evidence can guide safer designs, reveal limitations in long-term performance, and support decisions about advancing a bioengineering approach toward clinical applications.
In vivo studies expose engineered solutions to the coordinated host processes that determine whether incorporation is stable and functional. By examining adhesion, matrix remodeling, vascularization, and immune regulation, researchers can identify design features that support safer and more durable performance. This host-construct information helps connect laboratory development with the requirements of potential clinical applications.