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In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice
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JoVE Journal Neuroscience
In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

DOI:
10.3791/51274-v

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11:38 min

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July 22, 2014

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, , , , , , ,
1Hans Berger Department of Neurology,Jena University Hospital, 2Immunology, Leibniz Institute for Age Research,Fritz Lipmann Institute, Jena, 3Institute of Diagnostic and Interventional Radiology, Medical Physics Group,Jena University Hospital

Chapters

  • 00:05Title
  • 02:41Intravitreal Manganese Injection
  • 04:32Preparing Animals for the MRI
  • 07:25MRI Data Analysis
  • 08:45Results: Contrast-enhanced MR Imaging
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

This video illustrates a method, using a clinical 3 T scanner, for contrast-enhanced MR imaging of the naïve mouse visual projection and for repetitive and longitudinal in vivo studies of optic nerve degeneration associated with acute optic nerve crush injury and chronic optic nerve degeneration in knock-out mice (p50KO).

Tags

Keywords: In Vivo ImagingOptic NerveMRIContrast EnhancementMEMRIRodent Visual SystemRetinal Ganglion CellsAxonal DegenerationAxonal RegenerationSynaptic PlasticityMnCl2NF-κB P50KO Mice

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