Lanthanide Donor
Lanthanide Donor - Related Videos
13:21
Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
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Cited by 9 •
2011
We demonstrate the metalation, purification, and characterization of lanthanide complexes. The complexes described here can be conjugated to macromolecules to enable tracking of these molecules using magnetic resonance imaging.
07:17
Single Port Donor Nephrectomy
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Cited by 5 •
2011
Single port laparoscopic surgery is changing the standard of care in surgical care like nothing since the laparoscopic technique was introduced 20 years ago. We present out technique of single port donor nephrectomy using the Gelpoint device. We have successfully performed this surgery in 100 patients.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
08:37
Corneal Donor Tissue Preparation for Endothelial Keratoplasty
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Cited by 31 •
2012
Endothelial corneal transplantation is a surgical technique for treatment of posterior corneal diseases. Mechanical microkeratome dissection to prepare tissue results in thinner, more symmetric grafts with less endothelial cell loss and improved outcomes. Dissections can be performed at the eye bank prior to corneal transplantation surgery.
12:52
Conjugation: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA First discovered by Lederberg and Tatum in 1946, conjugation is a form of horizontal gene transfer between bacteria that relies on direct physical contact between two bacterial cells (1). Unlike other forms of gene transfer, such as transformation or transduction, conjugation is a naturally occurring process in which...
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Their long-lived emission allows measurements to be collected after short-lived background fluorescence has decayed. Narrow-band emission also provides a more spectrally defined signal, helping distinguish donor or transferred acceptor light from unwanted optical contributions. Together, these properties improve the sensitivity of time-resolved fluorescence measurements used to detect weak biological signals.
When the donor and acceptor labels are sufficiently close, excitation of the lanthanide donor can produce emission from the acceptor through resonance energy transfer. Detecting that acceptor emission indicates that the labels occupy a nearby configuration. Because label proximity reflects molecular arrangement, the signal can serve as a readout of distance-related changes.
Europium and terbium are examples of lanthanide ions that generate long-lived, narrow-band emission. Their optical behavior supports time-resolved detection and helps reduce interference from short-lived background signals. Incorporating either ion into a luminescent molecule or ion complex therefore provides a donor signal suited to sensitive resonance energy-transfer measurements.
The assay begins by exciting the donor and then monitoring whether the nearby acceptor emits light. Detectable acceptor emission indicates that energy transfer occurred between the labels, while the extent of this response can provide a molecular proximity readout. This workflow converts an optical signal into information about interactions or structural relationships in a biological system.
Lanthanide donors support biosensor development, diagnostics, and quantitative analysis of biological processes. They are also useful for studying protein interactions and nucleic-acid interactions, where label proximity can report molecular association or changes in arrangement. The combination of sensitive emission and proximity-dependent transfer makes the approach adaptable to several measurement-focused bioengineering applications.
The measurements can reveal whether labeled molecules approach one another closely enough for resonance energy transfer, providing information about molecular interactions and spatial relationships. In protein and nucleic-acid studies, that proximity signal can help examine association-related behavior. In biosensors and diagnostics, it can support quantitative analysis rather than merely indicating that light was emitted.