Oxidized Rgd Conjugated Alginate Bio Ink
Oxidized Rgd Conjugated Alginate Bio Ink - Related Videos
16:38
Bridging the Bio-Electronic Interface with Biofabrication
0 Views •
Cited by 3 •
2012
This article describes a biofabrication approach: deposition of stimuli-responsive polysaccharides in the presence of biased electrodes to create biocompatible films which can be functionalized with cells or proteins. We demonstrate a bench-top strategy for the generation of the films as well as their basic uses for creating interactive biofunctionalized surfaces for lab-on-a-chip applications.
07:05
Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
0 Views •
Cited by 6 •
2015
A Cartesian bioprinter was designed and fabricated to allow multi-material deposition in precise, reproducible geometries, while also allowing control of environmental factors. Utilizing the three-dimensional bioprinter, complex and viable constructs may be printed and easily reproduced.
07:07
Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
0 Views •
Cited by 2 •
2015
The synthesis of asymmetric species of ferrocene is challenging using solution techniques. This report focuses on the methods carried out to produce a ferrocene-biotin bioconjugate using facile and clean reactions accomplished via solid-phase synthesis. Incorporation of a thiolate moiety is shown to impart the ability for immobilization on gold surfaces.
09:30
The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
0 Views •
Cited by 17 •
2016
We present a protocol for the synthesis of RGD-functionalized hydrogels as devices for cell and drug delivery. The procedure involves copper catalyzed alkyne-azide cycloaddition (CuAAC) between alkyne-modified polyacrylic acid (PAA) and a RGD-azide derivative. The hydrogels are formed using microwave-assisted polycondensation and their physicochemical properties are investigated.
10:49
Planar and Three-Dimensional Printing of Conductive Inks
0 Views •
Cited by 58 •
2011
Planar and three-dimensional printing of conductive metallic inks is described. Our approach provides new avenues for fabricating printed electronic, optoelectronic, and biomedical devices in unusual layouts at the microscale.
FAQs
Oxidation adds reactive aldehyde groups to the alginate backbone. These groups alter the polymer’s chemical functionality and contribute to the material’s tunable properties, while the alginate still provides the network-forming component of the hydrogel. In bioengineering, this modification helps create a printable matrix whose chemistry can be adjusted for cell-laden tissue-like constructs.
The arginine-glycine-aspartic acid, or RGD, sequence supplies integrin-binding sites that alginate alone does not provide in the described system. Integrins are cell-surface adhesion receptors, so presenting RGD can improve how encapsulated cells interact with the surrounding hydrogel. This biological interaction is relevant when the printed construct must support a more tissue-like cellular microenvironment.
Divalent ions such as calcium crosslink the alginate chains after printing, creating a more stable hydrogel network. Crosslinking helps the deposited material retain its structure and contributes to control over material properties. In practice, this step converts the printed, cell-containing formulation into a stabilized scaffold suitable for investigating tissue-like structures and engineered tissue models.
The two modifications address different design requirements. Oxidation introduces aldehyde-containing chemistry that supports tunable material behavior, whereas RGD functionalization improves biological interaction through integrin binding. Combining them allows one alginate-based matrix to address both structural and cellular considerations, which is useful when researchers need a printable scaffold that more closely represents aspects of a cellular microenvironment.
A general workflow begins by preparing the oxidized, RGD-conjugated alginate formulation with living cells, followed by depositing it through 3D bioprinting to create the intended tissue-like structure. After printing, calcium or another suitable divalent ion crosslinks the alginate network. The resulting construct is then evaluated as a cell-laden scaffold or engineered tissue model.
Researchers can tune the matrix’s material behavior through the alginate’s oxidation state, its RGD-mediated biological interaction, and the post-printing crosslinking provided by divalent ions. These factors influence how the scaffold stabilizes and how cells engage with it. Such tunability helps adapt printed constructs for different tissue-regeneration investigations and tissue-like model designs.
This material is useful when a study requires both 3D fabrication and improved cell interaction within the printed matrix. Applications include investigating tissue regeneration, constructing engineered tissue models, and developing printable scaffolds that better reproduce cellular microenvironments. Its value comes from combining a stabilizable alginate network with RGD-mediated adhesion in a cell-laden format.