Perfusable Microchannel
Perfusable Microchannel - Related Videos
10:55
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
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Cited by 5 •
2013
A microchannels-on-a-chip platform was developed by the combination of photolithographic reflowable photoresist technique, soft lithography, and microfluidics. The endothelialized microchannels platform mimics the three-dimensional (3D) geometry of in vivo microvessels, runs under controlled continuous perfusion flow, allows for high-quality and real-time imaging and can be applied for microvascular research.
10:24
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
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Cited by 16 •
2016
Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
03:44
Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
FAQs
Flow is established by applying a pressure difference or using a pump to move culture medium through the channel. Because the conduit is microscale, researchers can adjust flow rate with relatively fine control and thereby regulate transport conditions, including shear stress and the delivery of dissolved factors. These variables make experiments more reproducible.
Channel dimensions are central experimental variables because they influence how quickly medium moves and how strongly flowing liquid acts on nearby cells. Researchers therefore treat flow rate, shear stress, and dissolved-factor exposure as linked conditions rather than isolated settings. Controlling them allows bioengineers to test how cells respond to transport environments that more closely resemble biological systems.
Continuous perfusion repeatedly supplies fresh culture medium and carries away accumulated waste. This improves mass transfer compared with relying only on local exchange around a culture, while maintaining defined flow conditions. The resulting setup can reveal responses to sustained transport and exposure patterns that may be missed when delivery and removal are not continuously controlled.
An experimental setup typically couples a microchannel to a source of culture medium and a mechanism for driving flow, such as a pump or imposed pressure difference. Researchers then establish the desired flow conditions and maintain medium passage through the channel. This workflow supports controlled delivery, waste removal, and exposure to dissolved factors during a bioengineering experiment.
Bioengineers use these channels to reproduce transport through blood-vessel-like environments, connect engineered tissues, and build organ-on-a-chip platforms. In each case, perfusion adds a controllable transport component to the model rather than leaving cells or tissues dependent on static exposure. The channel can therefore help link fluid movement with tissue function, disease-related processes, or treatment responses.
Measurements from a perfused system can be used to examine cell responses, tissue function, disease processes, and therapeutic performance under defined transport conditions. The value lies not only in observing an endpoint, but also in relating that outcome to flow rate, shear stress, and dissolved-factor exposure. This connection helps researchers interpret how transport conditions shape biological behavior.