Rrna Fish Probes
Rrna Fish Probes - Related Videos
12:16
Robust 3D DNA FISH Using Directly Labeled Probes
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Cited by 49 •
2013
We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.
11:15
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
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Cited by 16 •
2015
Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.
07:25
Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria
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Cited by 2 •
2017
A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.
02:32
Osmoregulation in Fishes
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2019
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments? Fish employ osmoregulatory strategies to balance bodily levels of water and dissolved ions (i.e., solutes), such as sodium and chloride. Imagine two solutions separated by a membrane that is permeable to water.
10:57
16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species
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2023
Source: Ewa Bukowska-Faniband1, Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Planet Earth is a habitat for millions of bacterial species, each of which has specific characteristics. Identification of bacterial species is widely used in microbial ecology to determine biodiversity of environmental samples and medical microbiology to diagnose infected patients. Bacteria can be classified...
FAQs
Detection depends on the probe recognizing a complementary sequence within ribosomal RNA. A probe therefore provides identification at the species or group level according to the rRNA sequence it targets. This sequence-based selectivity allows researchers to distinguish particular members of a microbial community while examining their locations within the same preserved sample.
Fixation preserves the sample and its spatial organization, while permeabilization helps the fluorescently labeled oligonucleotides reach accessible rRNA inside cells or microorganisms. These preparation steps connect molecular recognition with spatial observation. If rRNA remains inaccessible, probe binding and the resulting fluorescence may not adequately represent the cells present in the sample.
Hybridization occurs under controlled temperature and salt conditions that support binding between the probe and its complementary rRNA sequence. These conditions are important because probe attachment depends on the interaction between the two nucleic acid sequences. Controlling them helps produce interpretable fluorescence patterns for identifying targeted cells or microbial groups.
Fluorescence reveals where probe-bound cells or microorganisms are located within the preserved sample. The resulting microscopy image can therefore connect identity with distribution rather than reporting only that a target is present. This spatial information is especially useful for examining organization in microbial communities, bioengineered tissues, and bioreactor systems.
A typical workflow begins by fixing the sample and permeabilizing its cells or microorganisms. Fluorescently labeled probes are then introduced under controlled temperature and salt conditions so they can hybridize with accessible rRNA. Finally, microscopy detects the fluorescence from bound probes, allowing researchers to identify targets and evaluate their spatial distribution.
In bioengineering, these probes can profile microbial communities and visualize cell distribution in bioengineered tissues, bioreactors, and other complex systems. Their value comes from retaining spatial context while identifying selected cells or microorganisms. Researchers can therefore examine not only community composition, but also where particular microbial groups occur within an engineered environment.