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Journal / Biology / Robust 3D DNA FISH Using Directly Labeled Probes
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Robust 3D DNA FISH Using Directly Labeled Probes

Article DOI: 10.3791/50587 • 12:16 min • August 15th, 2013
August 15th, 2013 •
1, 2, 2,3, 1, 2
1Nuclear Dynamics Programme, The Babraham Institute, 2Epigenetics Programme, The Babraham Institute, 3Centre for Trophoblast Research, University of Cambridge

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Summary
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We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.

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3D DNA FISH Directly Labeled Probes Protocol Robustness Reproducibility Ease Of Use Nick-translation Amino-allyldUTP Chemical Coupling Fluorescent Probes Freeze-thaw Step Cell Permeabilization Heating Step Denaturation High-throughput Automated Imaging Chromosomal Regions
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