Chip Functionalization
Chip Functionalization - Related Videos
06:34
Synthesis of Antiviral Tetrahydrocarbazole Derivatives by Photochemical and Acid-catalyzed C-H Functionalization via Intermediate Peroxides (CHIPS)
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Cited by 1 •
2014
A two-step procedure for the synthesis of pharmaceutically active indole-derivatives by C-H functionalization with anilines is described, using photo- and Brønsted acid catalysis.
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
12:43
On-Chip Endothelial Inflammatory Phenotyping
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Cited by 3 •
2012
Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.
FAQs
These surface properties regulate how biological samples interact with the chip. Wettability can affect contact with the interface, charge can influence interactions with sample components, and molecular recognition determines whether an immobilized probe selectively binds its target. Together, they shape capture efficiency, sample transport, and signal generation, making surface-property control central to analytical performance.
Adsorption and covalent coupling are two routes for placing biological probes at the chip interface. Both can immobilize antibodies, enzymes, nucleic acids, or aptamers, but they represent different attachment strategies for constructing the functional surface. Selecting between them is part of matching the interface design to the intended biological interaction and assay conditions.
Probe activity determines whether the functionalized interface can recognize or interact with the intended biological target. Antibodies, enzymes, nucleic acids, and aptamers therefore need conditions that preserve their function during the assay. Maintaining activity supports reliable capture and signal generation, which directly affects the selectivity and analytical performance of biological measurements.
Planning should account for the chip surface or internal structures, the chosen chemical groups, polymers, or biomolecular probes, and the assay conditions in which the interface will operate. The design should also consider whether the goal is capture, transport, or signal generation. These decisions help align the engineered interface with the intended biological technique.
A functionalized chip places recognition elements such as antibodies, enzymes, nucleic acids, or aptamers where they can interact with biological samples. Controlled recognition can promote selective target capture and contribute to signal generation. In biosensors, this engineered interface supports analytical measurements by connecting molecular interactions at the chip surface with detectable assay performance.
For cell isolation, immobilized recognition probes can help create interfaces that interact selectively with components of a biological sample. In diagnostics, the same principle supports capture and signal generation at a chip-based interface. By controlling surface properties and probe activity, researchers can design systems that improve selectivity for biological analysis.
Functionalized interfaces provide a way to incorporate biological recognition into compact analytical platforms. In drug screening, engineered surfaces can support controlled interactions relevant to evaluating biological responses. In lab-on-a-chip systems, surface or internal modification helps manage interactions with samples while supporting capture, transport, and signal generation within integrated biological techniques.