Fraction Collector Harvesting
Fraction Collector Harvesting - Related Videos
07:11
Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification
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2026
An integrated autosampler and fraction collector (LC-mate) was developed to automate repetitive HPLC separations. Compatible with HPLC detectors that output analog signals, LC-mate enhances the performance of manually operated systems and accelerates research workflows that depend on high-performance liquid chromatography.
07:18
Experimental System of Solar Adsorption Refrigeration with Concentrated Collector
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Cited by 5 •
2017
With solar energy as the driving force, a novel adsorption refrigeration system has been developed and experimentally investigated. Water vapor and zeolite formed the working pair of the adsorption system. This manuscript describes the setup of the experimental rig, the operation procedure, and the important results.
10:56
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
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Cited by 217 •
2014
Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.
10:09
Fractional Distillation
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2023
Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation...
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
FAQs
Collection intervals determine how finely the eluate is divided. A time-based schedule advances the collector after preset periods, whereas a volume-based schedule changes positions after a specified amount of liquid has passed. Choosing between them affects how distinct eluting material remains across neighboring fractions and how easily researchers can identify or combine samples for later work.
Detector signals can coordinate collection with the appearance of separated material from the column. Instead of relying only on a fixed time or volume schedule, the collector can respond to information generated during the separation. This linkage helps associate collected fractions with observed separation behavior, supporting more informed selection of samples for testing or further processing.
Keeping successive fractions separate preserves differences in the material leaving the column. Individual samples can then be tested independently, compared with neighboring fractions, or selected for pooling when they show the desired separation pattern. This flexibility is important because collection connects the physical separation event with recoverable samples used in later biological experiments.
A typical setup places tubes or wells where the column eluate can be directed as it exits the separation system. The collector is configured to advance positions according to time or liquid volume, and it may also be linked to a detector. These settings establish how the outgoing stream becomes an organized series of recoverable samples.
Collected fractions can be retained for analysis or further processing rather than treated as a single combined stream. Depending on the experimental goal, researchers may test individual fractions, pool selected samples, or concentrate material before downstream experiments. The collection format therefore preserves options for evaluating and using separated biological components.
The approach is useful in chromatography workflows that separate proteins, nucleic acids, or other biological components. It allows the separated output to be recovered in an ordered set of samples, which can then support testing, pooling, concentration, or downstream experiments. Its value lies in linking column-based separation with practical sample recovery for biological analysis.