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JoVE Journal
Biology

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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
 

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Article doi: 10.3791/51455
May 17th, 2014

Summary May 17th, 2014

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

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