Interface Chamber Setup
Interface Chamber Setup - Related Videos
09:23
A Low Cost Setup for Behavioral Audiometry in Rodents
0 Views •
Cited by 17 •
2012
A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.
02:04
Protein-protein Interfaces
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2020
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
13:44
Sealable Femtoliter Chamber Arrays for Cell-free Biology
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Cited by 6 •
2015
A microfabricated device with sealable femtoliter-volume reaction chambers is described. This report includes a protocol for sealing cell-free protein synthesis reactants inside these chambers for the purpose of understanding the role of crowding and confinement in gene expression.
06:15
Rapid Setup of Tissue Microarray and Tiled Area Imaging on the Multiplexed Ion Beam Imaging Microscope Using the Tile/SED/Array Interface
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Cited by 2 •
2023
Multiplexed ion beam imaging (MIBI) is often used to image tissue microarrays and tiled, contiguous tissue areas, but current software for setting up these experiments is cumbersome. The tile/SED/array interface is an intuitive, interactive graphical tool developed to dramatically simplify and accelerate MIBI run setup.
07:49
Development of an Experimental Setup for the Measurement of the Coefficient of Restitution under Vacuum Conditions
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Cited by 11 •
2016
The coefficient of restitution is a parameter that describes the loss of kinetic energy during collision. Here, a free-fall setup under vacuum conditions is developed to be able to determine the coefficient of restitution parameter for particles in micrometer range with high impact velocities.
FAQs
The oxygenated culture medium or artificial cerebrospinal fluid supplies nutrients from below while carrying away metabolic waste. At the exposed surface, humidified gas helps maintain the local environment without allowing the tissue to dry. Together, these two phases support living excised tissue during measurements and help preserve conditions needed to study cellular signaling, network activity, and physiology.
The porous support physically holds the tissue while allowing the underlying medium to contact its lower surface. This arrangement separates the tissue from direct immersion but preserves access to flowing, oxygenated fluid. Its position therefore helps maintain the air–liquid configuration required for controlled ex vivo experiments involving organotypic cultures, electrophysiology, microscopy, or tissue physiology.
Stable environmental control helps keep the specimen viable long enough to obtain meaningful measurements. Consistent access to oxygenated medium, waste removal, and humidified gas reduces changes that could interfere with cellular signaling, network activity, or physiological responses. This stability is particularly relevant when researchers compare tissue behavior across imaging, electrophysiological, or treatment-response experiments.
A typical preparation places the excised tissue on a porous support, positions oxygenated culture medium or artificial cerebrospinal fluid beneath it, and exposes the upper surface to humidified gas. The arrangement is then maintained under controlled environmental conditions so fluid delivery, waste removal, and tissue exposure remain consistent during the planned measurements.
This configuration supports organotypic culture work, brain-slice electrophysiology, microscopy, and tissue physiology experiments. It is useful when investigators need to observe living tissue ex vivo while controlling its immediate environment. Depending on the study, measurements may focus on cellular signaling, network activity, physiological behavior, or responses to experimental treatments.
Measurements can reveal how living tissue behaves under controlled ex vivo conditions, including changes in cellular signaling, network activity, and tissue physiology. The setup also permits assessment of responses to experimental treatments while the specimen remains supported by oxygenated medium and humidified gas. These capabilities connect chamber-based observations with broader questions in biological techniques.