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Sealable Femtoliter Chamber Arrays for Cell-free Biology
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Sealable Femtoliter Chamber Arrays for Cell-free Biology

Sealable Femtoliter Chamber Arrays for Cell-free Biology

DOI:
10.3791/52616-v

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13:44 min

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March 11, 2015

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, , , , , ,
1Bredesen Center,University of Tennessee, Knoxville, 2Center for Nanophase Materials Sciences,Oak Ridge National Laboratory, 3Department of Materials Science and Engineering,University of Tennessee, Knoxville

Chapters

  • 00:05Title
  • 01:08PDMS Device Fabrication
  • 05:47Cell-free Protein Synthesis: Microfluidic Connections Setup
  • 08:25Cell-free Protein Synthesis: Sample Loading and Data Acquisition
  • 11:00Image Analysis and Data Processing
  • 11:58Results: Stochastic Processes in Gene Networks Can Be Assayed Using Femtoliter Cell-free Protein Synthesis Reactions
  • 13:19Conclusion

Summary

Automatic Translation

Automatic Translation

A microfabricated device with sealable femtoliter-volume reaction chambers is described. This report includes a protocol for sealing cell-free protein synthesis reactants inside these chambers for the purpose of understanding the role of crowding and confinement in gene expression.

Tags

Cell-free BiologyFemtoliter ChambersPDMS DeviceCell-free Protein SynthesisReaction KineticsGene CircuitsNoise Biology

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