Laser Activation
Laser Activation - Related Videos
06:32
A Chitosan Based, Laser Activated Thin Film Surgical Adhesive, 'SurgiLux': Preparation and Demonstration
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Cited by 32 •
2012
The fabrication of a novel, flexible thin film surgical adhesive from FDA approved ingredients, chitosan and indocyanine green is described. Bonding of this adhesive to collagenous tissue through a simple activation process with a low-powered infra-red laser is demonstrated.
07:07
Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion
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Cited by 13 •
2013
Here we describe a protocol for optogenetic manipulation of motoneuronal activity while monitoring changes in motor output (muscle contraction) in semi-intact Drosophila larvae using lasers within a conventional confocal microscope. This technique enables researchers to achieve local perturbation of neural activity in a few neuromeres to elucidate the dynamics of motor circuits.
06:27
Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
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Cited by 13 •
2017
The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.
14:18
Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
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Cited by 2 •
2016
A protocol to detect and automate mode locking in a pre-adjusted nonlinear polarization rotation fiber laser is presented. The detection of a sudden change in the output polarization state when mode locking occurs is used to command the alignment of an intra-cavity polarization controller in order to find mode-locking conditions.
11:00
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells
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2019
Tightly-focused femtosecond laser can deliver precise stimulation to cells by being coupled into a confocal microscopy enabling the real-time observation and photostimulation. The photostimulation can activate cell molecular events including ERK signaling pathway and mitochondrial flashes of reactive oxygen species.
FAQs
The absorbed wavelength is the first determinant of how laser activation affects a target. Photons must match an appropriate wavelength for absorption, after which their energy may drive a photochemical reaction, produce local heating, or release a light-sensitive compound. Thus, wavelength selection links the optical input to the type of event being studied.
Intensity and exposure duration provide adjustable control over the amount and timing of delivered light. Changing these settings can help researchers tailor activation to a particular biological system rather than applying one fixed condition. Their adjustment is especially important when experiments require precise timing or when nearby structures should remain largely unaffected.
These responses differ in what absorbs the photon energy and how that energy produces an effect. Photochemical activation changes a chemical process, local heating raises temperature in the illuminated region, and light-triggered release makes a previously contained compound available. Selecting among these mechanisms allows the same optical approach to address different experimental objectives.
A typical workflow begins by identifying the target and choosing a wavelength that the relevant material can absorb. The researcher then focuses the light on the selected location and adjusts intensity and duration to suit the desired event. This controlled exposure can activate molecules, cells, or subcellular structures while limiting effects outside the illuminated region.
Researchers can use this approach when an experiment requires activation at a defined location and time. It supports investigations of signaling, molecular interactions, and cellular dynamics, where broad stimulation could obscure local responses. The ability to manipulate cells or subcellular structures selectively makes the method useful for connecting a controlled intervention with a specific biological outcome.
Laser activation can reveal how a biological system responds when a molecule, cellular region, or subcellular structure is manipulated under controlled optical conditions. By varying wavelength, intensity, or duration, researchers can examine differences in activation and timing. These observations help relate localized experimental interventions to signaling behavior, molecular interactions, and cellular dynamics.