Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells

Shaoyang Wang; Minglie Hu; Tao Ren; Hao He

doi:10.3791/59661

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Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells

JoVE Journal
Biology

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JoVE Journal Biology

Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells

6,760 Views

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11:00 min

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July 06, 2019

DOI:

10.3791/59661-v

DOI:

10.3791/59661-v

•

11:00 min

July 06, 2019

•

6754 Views

Shaoyang Wang^1,2, Minglie Hu², Tao Ren¹, Hao He³

¹Department of Respiratory Medicine, Shanghai Sixth People’s Hospital, School of Medicine,Shanghai Jiao Tong University ²Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering,Tianjin University ³School of Biomedical Engineering,Shanghai Jiao Tong University

Summary

Tightly-focused femtosecond laser can deliver precise stimulation to cells by being coupled into a confocal microscopy enabling the real-time observation and photostimulation. The photostimulation can activate cell molecular events including ERK signaling pathway and mitochondrial flashes of reactive oxygen species.