Radioactive Co2 Trapping
Radioactive Co2 Trapping - Related Videos
04:25
Substrate Oxidation Assay in Cultured Cells: An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO2
0 Views •
2025
This video demonstrates a quantification technique for CO2 released during substrate oxidation using 14C-radiolabeled substrates. The released 14CO2 is trapped in an alkaline solution and quantified by a scintillation counter. The oxidation of different substrates varies between tissues and reflects the pathophysiological condition of the tissue.
03:18
Radioactivity and Nuclear Equations
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2020
Nuclear chemistry is the study of reactions that involve changes in nuclear structure. The nucleus of an atom is composed of protons and, except for hydrogen, neutrons. The number of protons in the nucleus is called the atomic number (Z) of the element, and the sum of the number of protons and the number of neutrons is the mass number (A). Atoms with the same atomic number but different mass numbers are isotopes of the same element. A nuclide of an element has a specific number of protons and...
02:48
Radioactive Decay and Radiometric Dating
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2020
Radioactivity is a spontaneous disintegration of an unstable nuclide and is a random process, as all the nuclei in the sample do not decay simultaneously. The number of disintegrations per unit time is called the activity (A), which is directly proportional to the number of nuclei in the sample. The decay constant (λ) is an average probability of decay per nucleus in unit time. The SI unit for activity is the becquerel, which is one disintegration per second. Another unit of activity is the...
14:17
Neutrophil Extracellular Traps: How to Generate and Visualize Them
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Cited by 296 •
2010
Neutrophil Extracellular Traps (NETs) are an important innate immune mechanism to fight pathogenic bacteria, fungi and parasites. Here we describe methods to isolate neutrophil granulocytes from human blood and to activate them to form NETs. We present preparation techniques to visualize NETs in light and electron microscopy.
01:41
Social Traps
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2020
Social traps are negative situations where people get caught in a direction or relationship that later proves to be unpleasant, with no easy way to back out of or avoid. The concept was orignally introduced by John Platt who applied psychology to Garrett Hardin's "Tragedy of the Commons", where in New England herd owners could let their cattle graze in the common ground. This situation seems like a good idea, but an individual could have an advantage. If they owned more cows, the larger...
FAQs
Radioactively labeled carbon dioxide provides a detectable signal that follows carbon after it enters a biological reaction. When the reaction is stopped, the labeled carbon can be separated or trapped, and its radioactivity quantified. The measured signal therefore links carbon dioxide assimilation to the experimental condition, allowing otherwise difficult-to-follow fixation or metabolic activity to be compared.
Defined conditions give the radioactivity measurement an interpretable reference. Because labeled carbon dioxide is supplied under specified conditions, differences in recovered radioactivity can be related to changes in biological activity rather than treated as an isolated signal. This supports comparisons among experiments and helps identify pathway changes associated with carbon processing.
By following labeled carbon, the method can be applied to several carbon-processing contexts rather than one pathway alone. The relevant uses include photosynthesis, respiration, carbon fixation, and metabolic flux. In each case, radioactivity provides a measurable connection between the supplied carbon dioxide and the biological activity being investigated.
Separation or trapping helps associate the measured radioactivity with carbon that has been incorporated or retained by the reaction system. Stopping the reaction first preserves the experimental endpoint, while subsequent handling makes the labeled carbon available for quantification. This sequence improves the connection between the signal and the carbon-assimilation event being studied.
A typical workflow begins by supplying radiolabeled carbon dioxide to cells, tissues, or an isolated reaction under defined conditions. The reaction is then stopped at the selected endpoint. Labeled carbon is separated or trapped, after which radioactivity is quantified. Researchers can relate that measurement to carbon dioxide assimilation and compare results across conditions.
The assay can be adapted to cells, tissues, or isolated reactions, depending on the carbon-processing question. Using these different experimental systems allows researchers to examine activity in an intact biological sample or in a more specifically defined reaction. The choice of system helps connect measured carbon assimilation with the process or mechanism under investigation.
Within biological techniques, the method supplies quantitative evidence for carbon-processing activity that may be difficult to follow directly. Researchers can compare biological activity under different experimental conditions, investigate pathway changes, and characterize carbon-processing mechanisms. Its value comes from linking a measurable radioactivity signal to the conditions and system in which carbon dioxide is assimilated.