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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Neutrophil Extracellular Traps
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Neutrophil Extracellular Traps: How to Generate and Visualize Them

Article DOI: 10.3791/1724
February 24th, 2010 •
1, 1, 1,2, 1,2, 2
1Core Facility Microscopy, Max Planck Institute for Infection Biology, 2Cellular Microbiology, Max Planck Institute for Infection Biology

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February 24th, 2010

Neutrophil Extracellular Traps (NETs) are an important innate immune mechanism to fight pathogenic bacteria, fungi and parasites. Here we describe methods to isolate neutrophil granulocytes from human blood and to activate them to form NETs. We present preparation techniques to visualize NETs in light and electron microscopy.

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