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JoVE Journal
Immunology and Infection

This content is Open Access.

Neutrophil Extracellular Traps

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Article DOI: 10.3791/1724
February 24th, 2010


Summary February 24th, 2010

Neutrophil Extracellular Traps (NETs) are an important innate immune mechanism to fight pathogenic bacteria, fungi and parasites. Here we describe methods to isolate neutrophil granulocytes from human blood and to activate them to form NETs. We present preparation techniques to visualize NETs in light and electron microscopy.

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