Adventitious Infectious Agents
Adventitious Infectious Agents - Related Videos
14:34
Biocontained Carcass Composting for Control of Infectious Disease Outbreak in Livestock
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Cited by 10 •
2010
Using readily available materials, this biocontained composting system enables effective on-site disposal of large animal carcasses arising in the event of infectious disease outbreak. This procedure kills most infectious agents in carcasses and contaminated manure. Once infectious agent is confirmed non-viable, mature compost can be spread as fertilizer.
02:40
Amplification and Recovery of Infectious Bacteriophages
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2026
Source: Rustad, M. et al. Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System. J. Vis. Exp. (2017)This video demonstrates the amplification and recovery of infectious bacteriophages from E. coli cultures. It outlines the steps for plaque isolation, phage replication, controlled lysis, and purification.
11:27
Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging
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Cited by 5 •
2016
This protocol describes the preparation and characterization of a dendrimeric magnetic resonance imaging (MRI) contrast agent that carries cyclen-based macrocyclic chelates coordinating paramagnetic gadolinium ions. In a series of MRI experiments in vitro, this agent produced an amplified MRI signal when compared to the commercially available monomeric analogue.
10:19
Reducing Agents
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2023
Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA Controlling the reactivity and selectivity during the synthesis of a molecule is very important criteria for chemists. This has led to the development of many reagents that allow chemists to pick and choose reagents suitable for a given task. Quite often, a balance between reactivity and selectivity needs to be achieved. This experiment will use IR spectroscopy to monitor the reaction and to...
07:35
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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Cited by 15 •
2017
This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.
FAQs
Entry can occur through several points, including starting materials, reagents, equipment, personnel, and environmental exposure. Because these sources span both supplied materials and routine handling, contamination control cannot focus on a single step. Reviewing each potential route helps investigators identify where an unintended agent could be introduced and where surveillance should be applied.
Their ability to replicate in susceptible cells or persist without obvious signs makes them a significant interpretation problem. A culture may therefore appear normal while an unwanted agent remains present or biologically active. This possibility links surveillance to experimental validity because undetected contamination can undermine confidence in observations even when routine visual inspection reveals no clear warning.
Culture-based, molecular, and microscopic methods provide different forms of evidence, so surveillance can draw on more than one way to investigate contamination. Validation is important because it supports dependable use of a chosen method in the relevant system. Appropriate detection strategies strengthen decisions about contamination, product quality, and interpretation of experimental results.
A practical workflow begins by considering possible sources in starting materials, reagents, equipment, personnel, and the environment. Investigators then apply validated culture-based, molecular, or microscopic detection methods to the relevant cell culture, biological product, or laboratory system. The findings support judgments about experimental integrity, safety, purity, and consistency.
Evaluation is especially important in cell cultures, biological products, laboratory systems, vaccines, therapeutics, and other biologics. In these settings, an unintended agent can affect the reliability of research or raise concerns about product safety, purity, and consistency. Surveillance therefore serves both routine quality control and the assessment of biologically important materials.
In biology, surveillance helps distinguish genuine experimental findings from effects associated with unintended contamination. It supports experimental integrity by checking whether cultures and laboratory systems remain suitable for interpretation. The same activity also contributes to quality control and biosafety, while helping researchers evaluate the reliability and consistency of vaccines, therapeutics, and other biological products.