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Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging
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JoVE Journal Chemistry
Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

DOI:
10.3791/54776-v

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11:27 min

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December 04, 2016

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, , ,
1MR Neuroimaging Agents,Max Planck Institute for Biological Cybernetics

Chapters

  • 00:05Title
  • 01:10Preparation of the DOTA-type Macrocycle Monomer
  • 03:05Preparation of the Dendrimeric Chelator
  • 04:55Preparation of the Gd(III) Dendrimeric Constrast Agent (DCA)
  • 06:28In Vitro Characterization of a Dendrimeric Contrast Agent (DCA) and Monomeric Analogue (GdDOTA)
  • 09:09Results: Dendrimeric Contrast Agent Activity
  • 10:38Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the preparation and characterization of a dendrimeric magnetic resonance imaging (MRI) contrast agent that carries cyclen-based macrocyclic chelates coordinating paramagnetic gadolinium ions. In a series of MRI experiments in vitro, this agent produced an amplified MRI signal when compared to the commercially available monomeric analogue.

Tags

Keywords: DendrimerMagnetic Resonance ImagingContrast AgentParamagnetic ChelatingDO3ATert-butyl-2-bromo-4-nitrophenyl ButatnoatePalladium On Activated CarbonHydrogenationThiophosgeneDOTA-type Monomer

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