Aerobic Anaerobic Species
Aerobic Anaerobic Species - Related Videos
09:35
Enrichment Cultures: Culturing Aerobic and Anaerobic Microbes on Selective and Differential Medias
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2023
Source: Christopher P. Corbo1, Jonathan F. Blaize1, Elizabeth Suter1 1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301 Prokaryotic cells are able to inhabit nearly every environment on this planet. As a kingdom, they possess a great metabolic diversity, allowing them to use a wide variety of molecules for energy generation (1). Therefore, when cultivating these organisms in the lab, all necessary and specific molecules required to make energy must be...
02:15
Subculturing Sulfite-Reducing Anaerobic Bacteria from an Agar Culture
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2025
Source: Anwar, Z., et al. Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples. J. Vis. Exp. (2019).In this video, sulfite-reducing bacteria are cultured in a sulfite-containing agar medium, where they grow anaerobically and reduce sulfite to hydrogen sulfide, forming black precipitates with iron ions. Black colonies are isolated from the agar depth using aseptic techniques and transferred to fresh medium for continued anaerobic incubation.
11:13
Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
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Cited by 100 •
2013
Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile cultivation.
05:28
A Bacterial Infection Assay for Studying Anaerobic Bacteria-Host Cell Interactions
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2025
This video demonstrates a technique for assessing the ability of Porphyromonas gingivalis, an anaerobic pathogenic bacterium, to adhere to and invade human umbilical vein endothelial cells (HUVECs). After infecting the host cells with the bacteria, the host cells are lysed to release both adhered and internalized bacteria. When the lysate is plated in an appropriate medium, the presence of colonies indicates successful bacteria-host cell interactions.
07:34
Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
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Cited by 41 •
2014
A novel Upflow Anaerobic Solid State (UASS) reactor was used for biogas production from fibrous feedstock. Digestate from UASS reactor was hydrothermally carbonized into HTC biochar in a pressurized batch reactor. The integration of the two bioenergy concepts was applied in this study to increase overall bioenergy production.
FAQs
Their distribution follows the availability of molecular oxygen and the metabolic strategies each organism can sustain. Oxygen-rich settings favor organisms able to use oxygen during respiration, while oxygen-depleted locations support species that avoid oxygen or obtain energy through anaerobic pathways. This helps explain their presence in contrasting habitats, including soils, sediments, intestines, and oxygen-depleted water.
Obligate forms have a narrow relationship with oxygen, so their growth depends on conditions that match that requirement or tolerance. Facultative forms are more flexible and can adjust their energy-generating strategy when oxygen availability changes. This difference influences where each type can persist and makes facultative organisms especially suited to environments with fluctuating oxygen levels.
Both processes provide alternatives when organisms do not use oxygen in the same way as aerobic respiration, but they represent different metabolic strategies. Anaerobic respiration and fermentation allow life to continue under oxygen-limited conditions, helping explain how species persist in habitats where oxygen is absent or restricted. The chosen pathway contributes to ecological distribution and industrial usefulness.
Culturing requires conditions that reflect each organism’s relationship with oxygen. Aerobic forms need access to oxygen, whereas anaerobic forms require oxygen-limited conditions; facultative forms can be examined across changing oxygen availability. Comparing growth under these contrasting conditions helps researchers assess oxygen tolerance, identify metabolic flexibility, and relate laboratory behavior to environmental distribution.
They are examined when researchers need to understand how oxygen availability influences organisms associated with infection. Differences in oxygen tolerance and energy metabolism can help explain why related species occupy distinct sites, including oxygen-variable biological environments. This comparison supports the selection of culture conditions and improves interpretation of which organisms may be recovered from a sample.
Their contrasting oxygen requirements make them useful subjects in studies of soil, sediments, oxygen-depleted water, and animal intestines. The same metabolic differences also matter in biodegradation and fermentation, where oxygen availability influences which organisms can function. Comparing species therefore connects basic biology with environmental processes and industrial activities that depend on microbial metabolism.