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JoVE Journal
Immunology and Infection

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Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
 

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Article doi: 10.3791/50787
September 14th, 2013

Summary September 14th, 2013

Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile cultivation.

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