Angiogenic Effect
Angiogenic Effect - Related Videos
10:04
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
0 Views •
Cited by 12 •
2017
Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.
04:21
Generating Anti-Angiogenic Tumor-Associated Neutrophils
0 Views •
2025
This video demonstrates an assay to generate anti-angiogenic tumor-associated neutrophils (TANs). Upon taking a murine melanoma tumor containing pro-angiogenic TANs, the single cells are isolated using fluorescence-activated cell sorting (FACS) and treated with an inhibitor of nicotinamide phosphoribosyl transferase (NAMPT) to reduce the capacity to stimulate angiogenesis.
11:07
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
0 Views •
Cited by 3 •
2019
The aim of this method is to generate an in vivo model of tumor angiogenesis by xenografting mammalian tumor cells into a zebrafish embryo that has fluorescently-labelled blood vessels. By imaging the xenograft and associated vessels, a quantitative measurement of the angiogenic response can be obtained.
08:19
Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
0 Views •
Cited by 5 •
2019
Here, we show therapeutic potential of anti-angiogenic tumor-associated neutrophils after their transfer into tumor-bearing mice. This protocol can be used to manipulate neutrophil activity ex vivo and to subsequently evaluate their functionality in vivo in developing tumors. It is an appropriate model for studying potential neutrophil-based immunotherapies.
10:47
Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
0 Views •
Cited by 25 •
2019
This method describes the culture of iPSC-derived endothelial cells as 40 perfused 3D microvessels in a standardized microfluidic platform. This platform enables the study of gradient-driven angiogenic sprouting in 3D, including anastomosis and stabilization of the angiogenic sprouts in a scalable and high-throughput manner.
FAQs
An increase in vascular endothelial growth factor, or VEGF, commonly signals that a cell, tissue, molecule, or treatment may promote vascular growth. VEGF can activate endothelial cells, which then proliferate, migrate, remodel surrounding extracellular matrix, and organize into capillary-like structures. These coordinated responses provide a biological basis for evaluating how strongly an intervention influences vessel formation.
Endothelial-cell activation alone does not describe the entire response. Researchers also consider whether these cells proliferate, migrate, modify the extracellular matrix, and organize into capillary-like structures. Examining several behaviors helps connect molecular signaling with the physical development of vascular structures, making the assessment more informative than measuring a single cellular response.
Vascular growth may be promoted or suppressed depending on the biological activity of the molecule, treatment, or tissue being studied. Enhancement is relevant when new blood vessels could support tissue growth or repair, whereas inhibition is important when researchers seek to limit unwanted vascular development. This distinction allows the same general biological process to be examined for different therapeutic goals.
Evaluation can include changes in vascular endothelial growth factor signaling and the resulting endothelial-cell responses. Relevant observations include endothelial proliferation, migration, extracellular matrix remodeling, and organization into capillary-like structures. Together, these outcomes indicate whether a candidate produces coordinated vascular behavior, rather than merely altering one molecular signal without a corresponding cellular or structural response.
The measurement is useful when studying wound-healing responses, tissue engineering strategies, and therapeutic candidates. Researchers can use the observed vascular signals and endothelial behaviors to determine whether an intervention supports the vascular development associated with tissue growth or repair. This information helps clarify whether a candidate has the desired biological activity before considering its broader therapeutic relevance.
In tumor biology, assessing vascular growth helps researchers investigate how tumors establish blood supplies. Studying signals such as vascular endothelial growth factor and the associated endothelial responses can reveal whether a tumor-related environment promotes vessel development. The same context also supports evaluation of approaches intended to inhibit vascular growth, providing a basis for exploring therapies that target tumor-associated vascular support.