Angiogenic Potential
Angiogenic Potential - Related Videos
10:04
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
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Cited by 12 •
2017
Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.
08:19
Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
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Cited by 5 •
2019
Here, we show therapeutic potential of anti-angiogenic tumor-associated neutrophils after their transfer into tumor-bearing mice. This protocol can be used to manipulate neutrophil activity ex vivo and to subsequently evaluate their functionality in vivo in developing tumors. It is an appropriate model for studying potential neutrophil-based immunotherapies.
04:21
Generating Anti-Angiogenic Tumor-Associated Neutrophils
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2025
This video demonstrates an assay to generate anti-angiogenic tumor-associated neutrophils (TANs). Upon taking a murine melanoma tumor containing pro-angiogenic TANs, the single cells are isolated using fluorescence-activated cell sorting (FACS) and treated with an inhibitor of nicotinamide phosphoribosyl transferase (NAMPT) to reduce the capacity to stimulate angiogenesis.
06:50
Electric Potential
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2023
Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN Electric potential, also known as "voltage", measures the electric potential energy per unit charge. Electric field is a scalar quantity and is fundamental to many electrical effects. Like potential energy, what is physically meaningful is the difference in the electric potential. For example, the spatial variation in the electric potential is related to the electric...
00:52
Potential Energy
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2019
The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy. Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
FAQs
Vascular endothelial growth factor can initiate a sequence of endothelial-cell responses that includes activation, proliferation, migration, and organization into vessel-like networks. These responses provide progressively stronger evidence than any single cellular change alone. Examining the sequence helps researchers determine whether a molecule, tissue, cell, or biomaterial merely affects endothelial behavior or supports coordinated vessel formation.
Four behaviors are especially informative: endothelial-cell activation, proliferation, migration, and organization into vessel-like networks. Activation indicates that cells respond to a vascular signal, while proliferation and migration show expansion and movement. Network organization provides a later structural outcome. Considering these behaviors together gives a broader biological picture than measuring only one response.
New blood-vessel formation can support tissue growth and repair, but it can also contribute to disease progression. The same capacity therefore has different biological significance depending on context. In wound healing and development, increased vessel formation may be beneficial, whereas tumor vascularization or vascular disease may make abnormal angiogenic activity an important process to characterize or limit.
A study can assess angiogenic potential by examining endothelial-cell responses to a cell, tissue, molecule, or biomaterial. Measurements may focus on activation, proliferation, migration, and the organization of cells into vessel-like networks, often in relation to signals such as vascular endothelial growth factor. The resulting observations help characterize whether the tested material influences blood-vessel formation.
Researchers may evaluate a biomaterial or engineered tissue when determining whether it can support the blood supply needed for regenerative medicine or tissue development. The assessment can reveal effects on endothelial activation, growth, movement, and network organization. This information helps characterize whether the candidate promotes vascular integration or provides a basis for further investigation in engineered-tissue strategies.
Measurements are relevant to wound healing, embryonic development, tumor vascularization, and vascular disease. They also support studies of regenerative medicine, engineered tissues, and therapeutic candidates intended either to enhance blood supply or limit abnormal vessel growth. Comparing endothelial responses across these settings helps connect observed vessel-forming activity with the biological or therapeutic context being studied.