Cell Surface Detection
Cell Surface Detection - Related Videos
05:00
Localized Surface Plasmon Resonance Imaging to Detect Protein Secretions from a Single Cell
0 Views •
2025
This video demonstrates localized surface plasmon resonance imaging to detect protein secretions from a single cell. Using a microfluidic setup and a peptide-bound plasmonic nanostructure chip, changes in reflected light intensity at the resonance angle reveal interactions with secreted proteins from a single cell.
03:05
Detection of Vitronectin Binding to Bacterial Surfaces using Flow Cytometry
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2025
This video demonstrates the detection of vitronectin's interaction with bacterial surface proteins. The flow-cytometry analysis measures the fluorescence signal of fluorescently labeled antibodies bound to vitronectin, confirming its presence at the bacterial surface. This technique provides insights into host-pathogen interactions and potential bacterial infection therapies.
10:03
Swab Sampling Method for the Detection of Human Norovirus on Surfaces
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Cited by 40 •
2017
A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.
10:41
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
0 Views •
Cited by 14 •
2012
In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.
08:49
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Cited by 8 •
2020
This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions.
FAQs
Specificity comes from binding between an antibody or other molecular probe and a matching extracellular epitope, meaning a recognizable region exposed outside the cell membrane. This selective interaction allows researchers to associate a signal with a particular surface molecule rather than with the cell as a whole. The resulting marker pattern can help distinguish cellular identities or states.
Labels convert molecular binding into a detectable signal. Fluorescent labels can be observed through microscopy or measured in flow cytometry, while enzymatic labels support detection in related assays. Because the label is attached to the binding probe, its signal provides a way to identify or measure the abundance of selected molecules exposed on the cell surface.
Surface molecules can change when cells respond to environmental signals or disease-related conditions. Consequently, a marker pattern may indicate both which population a cell belongs to and what state it currently occupies. Tracking these changes connects alterations in membrane composition with cellular communication and function, making surface measurements useful for studying dynamic biological responses.
A basic workflow involves choosing a probe that recognizes the extracellular marker of interest, allowing that probe to bind the cell surface, and detecting the attached fluorescent or enzymatic label. The signal is then examined by microscopy, flow cytometry, or another related assay. This sequence links a selected membrane molecule to an observable measurement.
Surface markers are particularly useful when researchers need to separate or characterize different cell populations, such as during studies of differentiation or immune biology. Comparing marker patterns can reveal population-specific features and changes over time. The approach therefore supports investigations in immunology and developmental biology, where cellular identity and maturation are central research questions.
In cancer research, surface-marker measurements can help examine cellular identity, state, and responses associated with disease. In cell-based therapeutics, the same information can support characterization of cells before or during therapeutic development. More broadly, relating membrane composition to phenotype helps researchers evaluate whether cells display features relevant to their intended biological role.