Automated Focus Hold
Automated Focus Hold - Related Videos
01:25
Shrinkage, Obsolescence, and Holding Costs
0 Views •
2025
In inventory-based businesses, profitability often depends on more than just sales volume or procurement efficiency. Hidden costs tied to inventory management—specifically shrinkage, obsolescence, and holding costs—can significantly erode margins if left unmanaged.Shrinkage occurs when inventory is lost due to theft, damage, or administrative errors. Even with advanced tracking systems, discrepancies remain a persistent issue, especially in retail. Regular audits, employee training, and...
09:30
Focused Ion Beams
0 Views •
2023
Source: Sina Shahbazmohamadi and Peiman Shahbeigi-Roodposhti-Roodposhti, School of Engineering, University of Connecticut, Storrs, CT As electron microscopes become more complex and widely used in research labs, it becomes more of a necessity to introduce their capabilities. Focused ion beam (FIB) is an instrument that can be employed in order to fabricate, trim, analyze and characterize materials on mico- and nano-scales in a wide variety of fields from nano-electronics to medicine. FIB...
11:38
Voluntary Breath-hold Technique for Reducing Heart Dose in Left Breast Radiotherapy
0 Views •
Cited by 49 •
2014
The current priority in breast cancer radiotherapy is to reduce cardiac doses without compromising target tissue coverage. The voluntary breath-hold technique described here is a simple, inexpensive solution to this problem and capable of being instituted widely without the need for specialized equipment.
10:35
Bringing the Visible Universe into Focus with Robo-AO
0 Views •
Cited by 17 •
2013
Light from astronomical objects must travel through the earth's turbulent atmosphere before it can be imaged by ground-based telescopes. To enable direct imaging at maximum theoretical angular resolution, advanced techniques such as those employed by the Robo-AO adaptive-optics system must be used.
08:06
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
0 Views •
Cited by 1 •
2016
Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.
FAQs
Two control strategies are identified: the system can evaluate image focus directly or track a reference position. When drift appears, it commands a motorized objective or stage to change the focal position. This feedback process compensates for shifts associated with temperature changes, vibration, or sample movement, allowing sequential images to remain focused without relying on continuous manual adjustment.
The motorized objective or stage provides the physical adjustment that restores the selected focal plane. Monitoring alone cannot correct a change in focus; the mechanism must translate either the objective or the specimen relative to the imaging path. This coordinated sensing and movement is especially important during extended acquisition, when small disturbances can accumulate into visibly inconsistent images.
Manual refocusing can interrupt acquisition and may alter the imaging conditions around a live specimen. Automated correction maintains the focal position while the experiment proceeds, producing sharper and more consistent views across time points. That continuity supports measurements of changing cells, tissues, or fluorescent structures, where focus variation could complicate interpretation or reduce measurement reliability.
Temperature changes, vibration, and movement of the sample are the specific sources of drift identified for this function. Their effects can shift the focal relationship between the specimen and the microscope during acquisition. Automated Focus Hold addresses these disturbances by continuously monitoring focus or a reference position and applying motorized corrections, helping preserve a comparable focal plane throughout the recording.
During acquisition, the system monitors either image focus or a reference position, identifies a shift, and commands motorized objective or stage correction. The microscope then continues collecting images while the focal plane is stabilized. This workflow suits recordings in which refocusing by hand would interrupt the experiment or change conditions around cells, tissues, or fluorescent structures.
Long-term live-cell observation, developmental studies, and quantitative analysis are key applications. These experiments may require consistent views over extended imaging periods, while the specimen or surrounding conditions change. Stabilizing focus helps preserve image quality throughout the recording, supporting observation of cells, tissues, and fluorescent structures without repeated manual intervention.
Stable focus makes image features more consistently represented from one time point to the next. That consistency matters when investigators quantify cells, tissues, or fluorescent structures rather than merely inspect them visually. By reducing focus-related variation during acquisition, the function improves the reliability of measurements and supports comparisons across a time-lapse sequence.