Barley Embryo

The barley embryo is the developing plant within a barley grain, containing the tissues that give rise to the root, shoot, and first leaves during germination. As the seed absorbs water, the embryo resumes metabolism, activates stored nutrients through enzymes, and coordinates cell division and expansion; the scutellum transfers nutrients from the endosperm to the growing embryo, while the radicle and coleoptile emerge in sequence. Studying barley embryos reveals how cereals establish seedlings, regulate early development, and respond to environmental conditions. Embryo culture and germination assays also support research in plant physiology, genetics, crop improvement, and cereal biotechnology.

Barley Embryo - Related Videos

Research

JoVE Journal - Environment

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry

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2024

A method for metabolomic analysis of barley is presented. The method entails fractionation and derivatization of metabolites and analysis thereof by gas chromatography/mass spectrometry (GC/MS). Metabolomic analysis can be used to determine the effect of intercropping and drought stress on the grain.

Rabbit Embryo Thawing: A Warming Procedure to Recover Cryopreserved Rabbit Embryos for Embryo Transfer

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2025

This video demonstrates the procedure for thawing vitrified rabbit embryos and the precautionary measurement steps for optimal embryo recovery.

Microinjection of Live Drosophila Embryos: Early Delivery of Reagents to the Developing Embryo

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2023

This video describes how to perform microinjections that deliver reagents into live Drosophila embryos. Researchers can use this method to introduce exogenous compounds like nucleic acids and proteins during the early stages of embryo development. The featured protocol demonstrates how to set up and perform the procedure for injection of any soluble reagent–here, a fluorescent protein for live imaging experiments.

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

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2018

An improved protocol is presented for the measurement of transient gene expression from reporter constructs in barley aleurone cells after particle bombardment. The combination of automated grain grinding with 96-well plate enzyme assays provides high throughput for the procedure.

Prenatal Mouse Embryos Retrieval: A Procedure to Harvest Embryos from Pregnant Mouse

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2025

This video demonstrates the retrieval of embryos from a pregnant mouse. The recovered perinatal embryos can be used as model systems for studying mammalian development.

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