Binary Vector Plasmid
Binary Vector Plasmid - Related Videos
05:09
Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion
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2025
In this video, we demonstrate generation of transgenic Arabidopsis plants expressing stable transgene through Agrobacterium-mediated binary bacterial artificial chromosome-based gene transformation.
12:08
Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
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Cited by 4 •
2018
Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.
02:05
Establishing a Binary Culture of Saccharibacteria with Host Bacteria
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2025
Source: Collins, A. J., et al., Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity. J. Vis. Exp. (2021)This video demonstrates the establishment and maintenance of a binary culture of Saccharibacteria and its host bacterium by sequentially co-incubating them in host-specific growth medium.
08:16
Plasmid Purification
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2023
Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study. In this video, a step-by-step generalized procedure is described for how to perform plasmid purification.
01:26
Binary Fission
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2025
Binary fission is the primary mode of asexual reproduction in prokaryotes, such as bacteria. It results in the production of two genetically identical daughter cells. This highly efficient process ensures the rapid propagation of bacterial populations under favorable conditions and involves coordinated cellular and molecular events.DNA Replication and SeparationThe process begins with the replication of the bacterial chromosome. The circular DNA molecule unwinds at a specific origin of...
FAQs
The right and left T-DNA border sequences define the DNA segment intended for delivery into the plant cell. The helper plasmid supplies virulence proteins that recognize and process this bordered region, enabling its transfer during Agrobacterium-mediated transformation. Keeping the gene of interest and selectable markers within these boundaries helps distinguish the transferable genetic cargo from the remaining vector sequences.
The helper plasmid provides virulence proteins but does not supply the gene of interest described in the transfer construct. These proteins process the DNA located between the T-DNA borders and support its movement into the plant cell. This division of functions allows the transferable cargo and the machinery required for delivery to reside on separate plasmids.
Selectable markers help identify plant cells that have acquired the transferred T-DNA. Because the marker is positioned between the right and left border sequences, it can accompany the gene of interest during delivery and integration. Marker-based identification makes it possible to distinguish candidate transformed cells from cells that did not receive the intended genetic construct.
Expression depends on the successful transfer and integration of the T-DNA carrying the selected gene into the plant genome. Once integrated, the transgene can direct production of its encoded product in plant cells. Thus, experiments must consider both genetic delivery and the resulting expression outcome, rather than treating DNA transfer alone as evidence of functional transformation.
A typical workflow places the gene of interest and selectable markers between the T-DNA borders, uses Agrobacterium containing the separate helper plasmid, and applies the system to plant cells. Cells receiving the transferred DNA can then be identified through the selectable marker, while subsequent analysis determines whether the integrated transgene is expressed.
Binary vector plasmids support several plant biotechnology goals, including functional genomics, crop improvement, protein production, and development of genetically engineered plants. In functional genomics, an introduced gene can help examine gene activity or function. In applied work, the same transfer framework supports plants designed to produce selected proteins or display useful engineered traits.