Brdu Incorporation
Brdu Incorporation - Related Videos
03:24
BrdU Immunofluorescence Staining: A Technique to Identify Cells in Different Phases of Cell Cycle
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2023
This video describes the protocol for BrdU immunofluorescence staining. The technique allows the identification of cells in different phases of cell cycle. Addition of DNA dyes and antibody labeling allows the detailed analysis of the fate of the S phase cells at later times.
12:46
Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
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Cited by 6 •
2011
Environmental bacterioplankton are incubated with a model dissolved organic carbon (DOC) compound and a DNA labeling reagent, bromodeoxyuridine (BrdU). Afterward, DOC-degrading cells are separated from the bulk community based on their elevated BrdU incorporation using fluorescence activated cell sorting (FACS). These cells are then identified by subsequent molecular analyses.
09:14
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Cited by 4 •
2016
In this protocol, we describe a novel BrdU-ChIP-Slot-Western technique to examine proteins and histone modifications associated with newly synthesized or nascent DNA.
08:24
Bioinspired Soft Robot with Incorporated Microelectrodes
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Cited by 9 •
2020
A bioinspired scaffold is fabricated by a soft photolithography technique using mechanically robust and electrically conductive hydrogels. The micropatterned hydrogels provide directional cardiomyocyte cell alignment, resulting in a tailored direction of actuation. Flexible microelectrodes are also integrated into the scaffold to bring electrical controllability for a self-actuating cardiac tissue.
08:37
Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU
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Cited by 7 •
2020
This paper presents four of the most common techniques for visualizing BrdU positive cells to measure adult neurogenesis in rats. This work includes instructions for reagent preparation, thymidine analog administration, transcardial perfusion, tissue preparation, peroxidase immunohistochemical reaction, immunofluorescence, signal amplification, counterstaining, microscopy imaging, and cell analysis.
FAQs
BrdU labeling is tied specifically to S phase because that is when cells copy their DNA. As new DNA strands are produced, BrdU can replace thymidine and become part of the DNA, creating a molecular mark of replication. The proportion of labeled cells therefore provides information about ongoing cell-cycle activity rather than simply total cell number.
After labeling, specific antibodies identify the BrdU-containing DNA signal. The same underlying label can therefore be measured through immunostaining, enzyme-linked assays, or flow cytometry, with each format providing a way to quantify labeled cells or labeling levels. This flexibility supports proliferation analysis in both cultured cells and tissue samples.
The appropriate format depends on whether the study requires visual localization, assay-based measurement, or flow-based analysis. Immunostaining shows where the signal occurs in cells or tissues, enzyme-linked assays provide a quantitative assay format, and flow cytometry enables analysis of labeling across a cell population. The choice determines how replication-associated results are represented.
The substitution creates a detectable chemical distinction while allowing the analog to participate in DNA synthesis. Antibodies can then mark the newly synthesized DNA, linking a replication event to a measurable signal. This connection allows investigators to compare cell-cycle activity, proliferation, or replication-related changes between experimental conditions.
Cells or tissues are exposed to BrdU while DNA synthesis occurs, after which the incorporated analog is detected with specific antibodies. Researchers then select immunostaining, an enzyme-linked assay, or flow cytometry as the measurement format and quantify the resulting signal. This workflow connects labeling during replication with an interpretable proliferation readout.
Immunostaining is useful when the location of labeled cells within cultured material or tissue matters. An enzyme-linked assay suits studies centered on quantitative signal measurement, while flow cytometry is appropriate when labeling needs to be assessed across cells in a population. Each option preserves the same BrdU-based replication marker but emphasizes a different type of result.
BrdU incorporation supports studies that ask how cell-cycle activity changes during development, cancer biology, or tissue regeneration. It can also help assess how experimental treatments affect cellular growth or replication. By quantifying labeled cells or signal, researchers can compare proliferative responses across biological settings and treatment conditions.