Cardiac Puncture Technique
Cardiac Puncture Technique - Related Videos
04:47
Cardiac Blood Collection via Subclavian Puncture in Mice
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2025
Subclavian cardiac puncture is a simple, reliable method for collecting terminal blood in mice. It is easy to operate and ensures high-purity samples.
05:27
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
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Cited by 87 •
2007
Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.
11:53
Cecal Ligation Puncture Procedure
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Cited by 200 •
2011
The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.
06:29
Cardiac Magnetic Resonance for the Evaluation of Suspected Cardiac Thrombus: Conventional and Emerging Techniques
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Cited by 6 •
2019
The goal of this article is to describe how cardiac magnetic resonance can be used for the evaluation and diagnosis of a suspected cardiac thrombus. The method presented will describe data acquisition as well as the pre-procedure and post-procedure protocol.
09:13
Remote Magnetic Navigation for Accurate, Real-time Catheter Positioning and Ablation in Cardiac Electrophysiology Procedures
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Cited by 51 •
2013
This report provides a detailed description of a new remote navigation system based on magnetic driven forces, which has been recently introduced as a new robotic tool for human cardiac electrophysiology procedures.
FAQs
Gentle aspiration draws blood through the needle while limiting tissue disruption. This matters because controlled collection helps preserve sample quality and supports a more consistent procedure. Standardized aspiration is particularly important when specimens from different animals will be compared or when one sample will support hematology, serum or plasma analysis, immunology, and biochemical studies.
Cardiac puncture may access either a ventricle or an atrium, so the intended chamber must guide needle entry and animal positioning. The overview does not assign different analytical uses to blood from these chambers. Instead, careful targeting and consistent positioning help researchers obtain the intended sample while minimizing unnecessary tissue disruption.
A collected blood sample can support several levels of biological analysis. Hematology examines blood-related characteristics, while serum or plasma analysis provides material for additional measurements. The same collection approach can also contribute to immunology and biochemical studies, allowing researchers to investigate cellular, immune, or molecular aspects of laboratory-animal biology.
Reliability depends on more than successful needle placement. Appropriate anesthesia, accurate positioning, controlled entry into the selected chamber, gentle aspiration, and a standardized technique all influence the collection. Training and consistent execution reduce procedural variation, helping researchers obtain blood samples that are suitable for comparison and downstream biological analyses.
The procedure begins with appropriate anesthesia and positioning of the laboratory animal. A needle is then directed into the selected cardiac chamber, either the ventricle or atrium, and blood is drawn by gentle syringe aspiration. Because the method is generally terminal, the complete workflow must follow approved animal-welfare procedures and institutional requirements.
The collection setup requires a needle and syringe, along with preparation for appropriate anesthesia and animal positioning. Researchers must also establish a controlled, standardized workflow before needle entry. These preparations support accurate chamber access, reduce tissue disruption, and help ensure that the resulting blood sample remains suitable for the planned biological analyses.
Researchers may choose cardiac puncture when they need a relatively large blood sample from a small laboratory animal. The resulting specimen can support hematology, serum or plasma analysis, immunology, and biochemical studies. Its usefulness therefore extends across experiments that require sufficient blood for one or more complementary forms of biological assessment.
Training helps personnel perform anesthesia, positioning, chamber entry, and aspiration consistently, while institutional approval ensures that the procedure fits animal-welfare requirements. Since cardiac puncture is generally terminal, ethical oversight is central rather than optional. Following approved protocols protects animal welfare and strengthens the scientific reliability of samples collected for biology research.