Cd4+ Antibody
Cd4+ Antibody - Related Videos
08:20
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
0 Views •
Cited by 24 •
2016
This protocol describes the reproducible generation and phenotyping of human induced regulatory T cells (iTregs) from naïve CD4+ T cells in vitro. Different protocols for FOXP3 induction allow for the study of specific iTreg phenotypes obtained with respective protocols.
11:28
Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
0 Views •
Cited by 11 •
2016
The mechanisms that govern the interstitial motility of CD4 effector T cells at sites of inflammation are relatively unknown. We present a non-invasive approach to visualize and manipulate in vitro-primed CD4 T cells in the inflamed ear dermis, allowing for study of the dynamic behavior of these cells in situ.
10:00
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
0 Views •
Cited by 2 •
2016
We describe a standard antibody staining protocol for use in microscopy to determine the membrane expression and localization of gangliosides in resting and activated human naïve CD4+ T cells. Also described are real-time PCR experiments using <40,000 cells that do not require additional low input RNA kits.
06:39
Selective Isolation of CD4+ Vδ1+ γδ T Cells from Human Peripheral Blood Mononuclear Cells
0 Views •
2025
This video demonstrates a protocol for the magnetic labeling-based isolation of a low-concentration population of CD4+Vδ1+γδ T cells from human peripheral blood mononuclear cells (PBMCs). Using a combination of FITC-conjugated anti-Vδ1 antibodies, magnetic anti-FITC microbeads, and anti-CD4 antibody-coated superparamagnetic beads, the CD4+Vδ1+ T-cells can be successfully purified from the PBMC population.
02:40
Effect of an Immunomodulatory Pharmacological Agent on the Interaction Between Dendritic Cells and CD4+ T Cells
0 Views •
2025
This video demonstrates a method of converting dendritic cells (DCs) to tolerogenic DCs (TolDCs) using an immunomodulatory pharmacological agent. This conversion is confirmed by evaluating the TolDC function through their immunosuppressive effect on CD4+ T cells.
FAQs
The antibody binds a particular epitope, or molecular site, on the CD4 cell-surface glycoprotein. This selective interaction allows researchers to distinguish cells carrying the recognized CD4 marker from other cells in a sample. The resulting signal or attachment can support immune-population characterization, provided the antibody is suited to the intended detection, separation, or blocking application.
Antibody design and conjugation influence how CD4 recognition is translated into an experimental result. A reagent configured for labeling can support detection, whereas attachment to a solid support enables cell separation. Other designs can interfere with CD4-dependent interactions. Selecting the appropriate format therefore connects the same target specificity to different experimental goals and outcomes.
Some CD4+ antibodies are designed to block rather than simply identify cells. By binding the CD4 molecule, they can interfere with interactions that depend on this surface component. This use shifts the reagent from a descriptive tool to a functional probe, helping investigators examine the significance of CD4-dependent interactions in studies of T-cell function and immune responses.
CD4 labeling provides a way to characterize immune populations according to whether they carry the CD4 surface marker recognized by the reagent. In biology studies, that information helps researchers examine the presence or distribution of helper T lymphocytes and other CD4-associated immune cell populations. It can also support comparisons of immune composition across experimental conditions.
In flow cytometry, CD4+ antibody labeling supports detection and characterization of CD4-marked cells within a sample. Immunohistochemistry applies the same recognition principle to examine CD4-associated cells in a tissue context. These methods provide complementary views of immune populations, allowing researchers to study cellular composition and localization while matching the assay to the biological question.
A cell sample is exposed to a CD4+ antibody so that cells carrying the recognized marker become selectively labeled. The labeled population can then be directed through a cell-sorting procedure to separate it from other cells. This workflow is useful when investigators need a more defined immune-cell population for subsequent characterization or functional study.
Depletion or enrichment is appropriate when the experimental design requires changing the proportion of CD4-marked cells in a sample. Antibody attachment can help remove the targeted population or concentrate it, depending on the separation strategy. These approaches allow researchers to test how particular immune-cell populations influence broader immune responses or to obtain material enriched for further analysis.
CD4+ antibodies connect cell-surface recognition with measurable changes in immune-cell composition or behavior. Researchers can use them to characterize populations, examine T-cell function, and investigate immune responses in health and disease. Detection, sorting, enrichment, depletion, and blocking applications provide different experimental routes for relating CD4-associated cells to biological conditions.