Cell Culture Migration
Cell Culture Migration - Related Videos
07:44
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
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Cited by 9 •
2016
Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.
08:52
An Introduction to Cell Motility and Migration
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2023
Cell motility and migration play important roles in both normal biology and in disease. On one hand, migration allows cells to generate complex tissues and organs during development, but on the other hand, the same mechanisms are used by tumor cells to move and spread in a process known as cancer metastasis. One of the primary cellular machineries that make cell movement possible is an intracellular network of myosin and actin molecules, together known as “actomyosin”, which creates a...
08:39
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Cited by 27 •
2010
T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.
09:55
In vitro Cell Migration and Invasion Assays
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Cited by 627 •
2014
Commonly used, highly accessible methods for examining cell migration and invasion in vitro are described. The first method is the cell wound closure assay that measures cell motility. The second method is the transwell migration and invasion assay that assesses the chemotactic and invasive capacity of cells.
09:36
Study of Cell Migration in Microfabricated Channels
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Cited by 43 •
2014
A quantitative method to study spontaneous migration of cells in a one-dimensional confined microenvironment is described. This method takes advantage of microfabricated channels and can be used to study migration of large number of cells under different conditions in single experiments.
FAQs
External cues activate signaling pathways that reorganize the actin cytoskeleton, the internal filament system that supports cell shape and movement. At the same time, regulated cell-substrate adhesion anchors portions of the cell, allowing traction forces to develop. Coordinating these processes converts signaling into forward displacement across the laboratory substrate.
External cues determine how cells activate migration-related signaling pathways and reorganize their cytoskeleton and adhesions. Researchers can therefore compare movement under different chemical, physical, or genetic conditions. These comparisons help reveal which types of environmental or cellular changes alter the forces and behaviors associated with relocation.
Genetic conditions can modify the signaling pathways, actin organization, or cell-substrate adhesion processes that support movement. Comparing cells under different genetic backgrounds or manipulations allows researchers to associate altered migration with specific cellular regulatory changes. This approach connects observable relocation across a substrate with underlying mechanisms of cell behavior.
Scratch-wound, transwell, and chemotaxis experiments provide complementary ways to examine cultured-cell movement across or through a laboratory substrate. Using more than one assay can help researchers compare migration behavior under distinct experimental arrangements. Measurements from these models are especially useful when testing chemical, physical, or genetic effects on relocation.
This approach is useful when researchers need an in vitro model of relocation during development, wound repair, immune responses, or disease. Cultured-cell assays provide a controlled setting for comparing movement under defined conditions. They help connect cellular mechanics with broader biological processes without relying only on observations of whole tissues.
Migration assays can be applied to questions about cancer invasion, tissue regeneration, and the effects of drugs on cell behavior. By comparing movement under different conditions, researchers can determine whether a treatment or biological change is associated with altered relocation. The resulting data link measurable cellular movement with disease-related or regenerative processes.