Cfse Labeled Cells
Cfse Labeled Cells - Related Videos
09:49
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
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Cited by 39 •
2010
Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.
10:57
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The cell cycle is a universal process of life. During the cell cycle, a cell undergoes...
04:10
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
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Cited by 214 •
2010
CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.
11:54
Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
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Cited by 18 •
2012
This video protocol demonstrates the application of the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE) for the identification and separation of different sub-populations of cells in human glioblastoma based on frequency of cell division.
07:14
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
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Cited by 10 •
2016
An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.
FAQs
After the dye enters a cell, intracellular esterases remove its acetate groups and expose a reactive succinimidyl ester. That group forms covalent bonds with intracellular amines, creating a stable fluorescent signal within the labeled cell. This chemical retention allows researchers to follow the same cell population during later measurements instead of relying on transient dye uptake alone.
When a labeled cell divides, its fluorescent material is distributed between the two daughter cells, so each generation displays lower fluorescence than the preceding population. Flow-cytometric measurements can therefore show successive fluorescence levels that correspond to division histories. This makes the method useful for assessing how extensively a population has proliferated rather than recording only a final cell count.
Fluorescence measurements can help track changes in labeled populations over time, including their persistence, movement, and representation in experimental samples. A signal detected in a later sample may indicate that labeled cells remain present, while changes in where or how much signal is detected can support analyses of migration, survival, or population composition. Interpretation depends on the experimental context.
In mixed-cell cultures, the fluorescent signal provides a way to distinguish and follow the labeled population while other cells remain in the same experimental system. Researchers can examine division histories and population changes within that shared environment, supporting comparisons between responding and nonresponding cell groups. This is particularly relevant when cellular behavior depends on interactions among different populations.
A typical workflow begins by exposing cells to the membrane-permeable dye, allowing intracellular esterases to process it and intracellular amines to become covalently labeled. The cells are then followed over time in culture or an experimental tissue model. Subsequent flow-cytometric analysis measures fluorescence changes, allowing investigators to evaluate division and other population behaviors.
Flow cytometry measures fluorescence in individual cells, making it possible to compare signal intensity across the labeled population. Progressive decreases in fluorescence identify cells that have undergone successive divisions, while the presence of labeled cells in later measurements supports tracking over time. In mixed samples, these measurements can help assess population dynamics alongside experimental conditions such as immune activation.
Lymphocyte responses often involve changes in proliferation and population behavior, which CFSE measurements can resolve through generation-dependent fluorescence decreases. Researchers can use these division histories to evaluate how strongly lymphocytes respond under experimental conditions. The approach also supports analysis of mixed-cell cultures and tissue models, where immune activation may be examined together with survival, migration, or interactions among cell populations.