Chicken Embryo
Chicken Embryo - Related Videos
06:10
Gene Transfer into Older Chicken Embryos by ex ovo Electroporation
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Cited by 2 •
2012
A method of gene transfer into chicken embryos at later incubation stages (older than Hamburger and Hamilton stage (HH) 22) is described. This method overcomes disadvantages of in ovo electroporation applied to older chicken embryos and is a useful technique to study gene function and regulation at older developmental stages.
03:33
Visualization of Neural and Vascular Networks in a Chicken Embryo
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2025
Source: Delalande, J., et.al. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection. J. Vis. Exp. (2015)This video demonstrates the transplantation of a GFP-labeled donor neural tube from a stage-matched transgenic chicken embryo into a recipient embryo at the level of somites one to seven, followed by vascular labeling using a lipophilic fluorescent dye. The combined approach allows for direct...
13:06
In Ovo Electroporations of HH Stage 10 Chicken Embryos
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Cited by 19 •
2007
Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. Common applications of this technique include functional analysis of genes and putative enhancer elements. This video demonstrates neural tube electroporation in HH 10 chick embryos. Injection technique and proper egg handling are discussed.
15:42
Xenotransplantation of Human Stem Cells into the Chicken Embryo
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Cited by 31 •
2010
In this paper we present a method for transplanting human stem cells into various regions of the central nervous system of the chicken embryo. This provides an in vivo model for assessing the proliferation and differentiation of various types of human stem cells in embryonic tissue environments.
06:57
In ovo Electroporation of Chicken Embryos
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2023
Electroporation is a technique used in biomedical research that allows for the manipulation of gene expression via the delivery of foreign genetic material into cells. More specifically, in ovo electroporation is performed on early developing chicks (Gallus gallus domesticus) contained within their eggshells. In this procedure, DNA or knockdown constructs are first injected into a target tissue. However, the genetic material is unable to penetrate the plasma membrane to carry out its function...
FAQs
Gastrulation reorganizes the developing cells into three germ layers, creating the foundation for later tissue formation. These layers provide distinct developmental sources that are subsequently shaped through patterning, neurulation, and organogenesis. Studying their formation helps biologists connect early cellular organization with the emergence of tissues and organs during embryonic development.
These stages represent different levels of developmental organization. Patterning establishes the arrangement of body regions, neurulation shapes structures associated with the developing nervous system, and organogenesis produces recognizable organs. Separating them allows researchers to examine how an initially organized embryo progresses through coordinated changes in form and tissue development.
The yolk supplies nutrients while the extraembryonic membranes support gas exchange during incubation. Together, these structures provide essential support without being part of the embryo’s main body tissues. Their roles allow the developing organism to grow, organize its tissues, and proceed through successive developmental stages inside the egg.
Its development can be observed through the eggshell and examined at defined stages, making changes in form and organization easier to follow over time. This accessibility supports investigations of embryonic patterning, tissue formation, genetics, and environmental effects on growth. The model therefore connects visible developmental outcomes with underlying biological processes.
Stage-based examination can reveal how development changes from rapid cell division through gastrulation, patterning, neurulation, and organogenesis. Researchers can compare the organization and progression of tissues at different points rather than treating development as a single event. This approach helps identify when particular developmental processes occur and how they relate to one another.
The model provides a developmental system in which researchers can investigate genetics alongside environmental effects on growth. Observing embryos at defined stages makes it possible to relate altered developmental outcomes to processes such as tissue formation and embryonic patterning. These applications extend its value beyond morphology to broader questions about how development is regulated.