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Chapters
- 00:01Title
- 00:28Introduction
- 01:35Proper Egg Handling
- 02:23Windowing the Egg
- 04:05Injection and Electroporation
- 11:43Conclusion
Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. Common applications of this technique include functional analysis of genes and putative enhancer elements. This video demonstrates neural tube electroporation in HH 10 chick embryos. Injection technique and proper egg handling are discussed.
Tags
Ovo ElectroporationsChicken EmbryosDevelopmental BiologyGene RegulationGene FunctionDNA ConstructsRNA ConstructsGene ExpressionGene KnockdownReporter ConstructsFate MappingGene Regulatory ElementsChick ElectroporationQuick And Easy ExperimentsInexpensive ExperimentsWindow In EggshellEmbryo ManipulationVisualization Of EmbryoDNA InjectionFast Green Dye InjectionPlatinum ElectrodesElectrical PulsesPulse GeneratorEgg Storage And Handling
