Collagenase Elastase
Collagenase Elastase - Related Videos
10:23
Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase
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Cited by 56 •
2009
This video shows how to induce abdominal aortic aneurysms (AAA) in mice via transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta. The model has the ability to add broad insight into the pathobiology of AAA due to the emergence of numerous transgenic and gene knockout mice.
03:47
Developing an Experimental Aneurysm Model in a Rabbit Using an Elastase-Treated Vessel Pouch
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2025
Source: Wanderer, S., et al. Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit. J. Vis. Exp. (2020)This video demonstrates the creation of an experimental aneurysm model in a rabbit using an elastase-treated vessel pouch. After excising a segment of the right CCA, the pouch is treated with elastase, then sutured to the left CCA to restore blood flow. The resulting stress on the weakened pouch walls induces aneurysm formation.
09:41
Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
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Cited by 35 •
2014
Preclinical models of intracerebral hemorrhage are utilized to mimic certain aspects of clinical disease. Thus, mechanisms of injury and potential therapeutic strategies may be explored. In this protocol, two models of intracerebral hemorrhage are described, intrastriatal (basal ganglia) injections of autologous blood or collagenase.
07:09
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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Cited by 93 •
2013
A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.
08:54
Reliable Creation of a Murine Abdominal Aortic Aneurysm Model Using Elastin Sensitization and Perivascular Elastase
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2026
This protocol provides a repeatable and reliable method for inducing abdominal aortic aneurysms (AAAs) in C57BL/6 mice using elastin sensitization and perivascular elastase application, as an AAA model that incorporates an immune-sensitization component.
FAQs
These enzymes target complementary structural components of the extracellular matrix. Collagenase acts on collagen fibers, whereas elastase hydrolyzes elastin and can also act on other protein substrates. Their combined activity loosens the tissue framework more broadly than focusing on a single structural protein, which supports release of cells for subsequent biological analysis.
Breaking down the tissue framework separates cells from the structural environment that holds them together. This release makes cellular material accessible for primary isolation, culture, and experimental analysis. Because collagen and elastin contribute to tissue strength and elasticity, their controlled degradation also helps investigators examine how tissue structure relates to cellular behavior.
Enzyme concentration, exposure time, and temperature are the main controllable conditions identified for this digestion process. Increasing or extending treatment may improve tissue loosening, but the process must remain controlled to preserve cell viability and function. Adjusting these variables therefore determines whether cell release is efficient without compromising the resulting preparation.
Successful digestion is not measured only by how thoroughly the tissue framework is loosened. The released cells must also remain viable and retain function so they can support meaningful culture or analysis. Excessive or poorly controlled treatment can undermine that objective, whereas balanced conditions provide material suitable for studying cellular behavior after isolation.
The enzymes are applied under controlled concentration, exposure-time, and temperature conditions to loosen the tissue framework and enable cell release. The resulting cells can then be directed toward primary culture or experimental analysis. This workflow connects enzymatic tissue dissociation with downstream studies of cellular behavior, tissue organization, and cell properties.
Researchers use Collagenase Elastase when connective-tissue structure must be loosened to obtain cells for primary isolation or culture. The approach is also relevant when experiments require access to cells that remain embedded within tissue. By enabling release from the extracellular matrix, it supports analyses that would be difficult to perform on intact tissue alone.
Cells obtained through controlled enzymatic dissociation can support investigations of tissue organization and cellular behavior. After release, they may be used in primary culture or experimental analysis, allowing researchers to examine cells outside their original tissue framework. The usefulness of these outcomes depends on maintaining sufficient viability and function during digestion.