Herpesvirus Infection
Herpesvirus Infection - Related Videos
02:46
Intranasal Murine Herpesvirus Infection in Mice to Assess Host Immune Responses
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2026
Source: Zhang, J., et al. P. Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production. J. Vis. Exp. (2014)This video demonstrates intranasal delivery of murine herpesvirus in mice to induce a lung-specific immune response. Lung tissue is subsequently processed to obtain cytokine-containing supernatants for downstream assessment of host immune responses.
07:10
Establishment and Quantification of De Novo Lytic Infection by Cell-free Kaposi's Sarcoma-Associated Herpesvirus
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2025
This protocol describes an in vitro system to model de novo lytic infection of Kaposi's sarcoma-associated herpesvirus using BAC16-derived virions. The method enables investigation of early viral replication and dissemination. Infectious virus production is quantified by GFP-based infectious units assays and real-time PCR of encapsidated viral genomes.
02:41
Clonal Isolation and PCR Screening of GFP-Excised Recombinant Herpesvirus Retaining the Target Gene
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2026
Source: Tang, N., et al. Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing. J. Vis. Exp. (2019)This video demonstrates the clonal isolation and PCR-based screening of recombinant herpesvirus following site-specific excision of a GFP marker. Fluorescence-activated cell sorting is used to isolate non-fluorescent infected cells, and junction PCR confirms retention of the target gene in GFP-negative clones.
11:28
Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
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Cited by 2 •
2011
We describe a protocol to identify key roles of host signaling molecules in lytic replication of a model herpesvirus, gamma herpesvirus 68 (γHV68). Utilizing genetically modified mouse strains and embryonic fibroblasts for γHV68 lytic replication, the protocol permits both phenotypic characterization and molecular interrogation of virus-host interactions in viral lytic replication.
09:57
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
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Cited by 12 •
2016
Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.
FAQs
Cellular receptor binding initiates entry and enables the virus to deliver its genome into the host-cell nucleus. Once there, the viral genome directs production of viral proteins and progeny virions. This sequence connects a specific host-cell interaction with the later generation of infectious virus, making entry and nuclear delivery important stages for biological study.
Latency allows the viral genome to remain in host cells while viral gene expression is limited. Because the genome persists without continuous production of progeny virions, infection can continue beyond an initial episode. Studying this state helps biologists examine how persistent viral material is maintained and how limited expression relates to later disease.
Recurrence reflects reactivation, in which the virus resumes replication after a latent period. Changes in cellular or immune conditions can influence this transition, leading to renewed disease or enabling transmission. This mechanism makes reactivation central to understanding why herpesvirus infection may produce repeated clinical episodes rather than a single, permanently resolved event.
Herpesvirus infection can affect skin and mucous membranes as well as the nervous system, so its effects must be considered across different tissue contexts. This broad range allows researchers to examine how host cells and tissues respond to the same general viral process. It also connects local disease manifestations with persistent infection in the host.
A useful conceptual workflow follows receptor binding, genome delivery into the nucleus, viral protein production, formation of progeny virions, and possible entry into latency. Researchers can then examine whether changed cellular or immune conditions are associated with reactivation. Following these linked stages helps connect molecular events with persistence, recurrent disease, and transmission.
Diagnostic methods are relevant because they support investigation of herpesvirus infection as a biological process rather than only as a visible disease. In the broader research context, they can be considered alongside viral replication, latency, and reactivation. This connection helps biologists relate evidence of infection to host-pathogen interactions and changing disease states.
Herpesvirus infection provides a system for examining how viral replication, persistent genomes, limited gene expression, and reactivation interact with host biology. Research can therefore address immune control, diagnostic methods, and antiviral strategies within one infection model. These areas are connected because controlling disease requires understanding both viral behavior and the host conditions that influence it.